OBJECTIVE: To investigate the action of a red laser associated with a photosensitizer on the reduction of Enterococcus faecalis in dental root canal in vitro. STUDY DESIGN: Thirty prepared teeth with single canals were contaminated. The chemical group was irrigated with 0.5% NaOCl and left flooded for 30 minutes. In the laser group, a paste-based photosensitizer was maintained in the root canals for 5 minutes, and then irradiated with a laser at 685 nm using an optical fiber for an E of 1.8 J during 3 minutes. After treatment, the canal content was collected, serially diluted, and cultured to determine the number of colony-forming units. RESULTS: Photosensitizer alone or laser alone did not have any bactericidal effect. Chemical solution reduced viable bacteria in 93.25%. Laser photosensitization resulted in a reduction of 99.2%, a significantly higher bacterial reduction than NaOCl. CONCLUSION: Laser photosensitization was effective for reducing E. faecalis in root canals and could be an adjunct to endodontic treatment.
OBJECTIVE: To investigate the action of a red laser associated with a photosensitizer on the reduction of Enterococcus faecalis in dental root canal in vitro. STUDY DESIGN: Thirty prepared teeth with single canals were contaminated. The chemical group was irrigated with 0.5% NaOCl and left flooded for 30 minutes. In the laser group, a paste-based photosensitizer was maintained in the root canals for 5 minutes, and then irradiated with a laser at 685 nm using an optical fiber for an E of 1.8 J during 3 minutes. After treatment, the canal content was collected, serially diluted, and cultured to determine the number of colony-forming units. RESULTS: Photosensitizer alone or laser alone did not have any bactericidal effect. Chemical solution reduced viable bacteria in 93.25%. Laser photosensitization resulted in a reduction of 99.2%, a significantly higher bacterial reduction than NaOCl. CONCLUSION: Laser photosensitization was effective for reducing E. faecalis in root canals and could be an adjunct to endodontic treatment.
Authors: Aguinaldo S Garcez; Martha S Ribeiro; George P Tegos; Silvia C Núñez; Antonio O C Jorge; Michael R Hamblin Journal: Lasers Surg Med Date: 2007-01 Impact factor: 4.025
Authors: Aguinaldo S Garcez; Silvia C Núñez; Nilton Azambuja; Eduardo R Fregnani; Helena M H Rodriguez; Michael R Hamblin; Hideo Suzuki; Martha S Ribeiro Journal: Photomed Laser Surg Date: 2013-07-03 Impact factor: 2.796
Authors: Jacob Lee Fimple; Carla Raquel Fontana; Federico Foschi; Karriann Ruggiero; Xiaoqing Song; Tom C Pagonis; Anne C R Tanner; Ralph Kent; Apostolos G Doukas; Philip P Stashenko; Nikolaos S Soukos Journal: J Endod Date: 2008-04-25 Impact factor: 4.171
Authors: Aguinaldo S Garcez; Eduardo R Fregnani; Helena M Rodriguez; Silvia C Nunez; Caetano P Sabino; Hideo Suzuki; Martha S Ribeiro Journal: Lasers Med Sci Date: 2012-03-08 Impact factor: 3.161