Cloning of exotic/endangered species: desert bighorn sheep.

Cloning using somatic cell nuclear transfer (SCNT) may be a useful tool for conserving genetic diversity and for propagating exotic and/or endangered animal species. Somatic cells can be obtained easily, expanded in culture, cryopreserved, and thawed at a later date for use in NT. Significant challenges relevant to using SCNT for cloning wild and endangered animal species include the need for using interspecies NT and interspecies embryo transfer. Animal care and welfare issues raised that are unique to exotic and endangered species also are raised. In this chapter, the methods used in attempts to clone the wild animal species of Desert Bighorn Sheep are described.