Literature DB >> 16985238

Simultaneous optical measurements of cytosolic Ca2+ and cAMP in single cells.

Mark C Harbeck1, Oleg Chepurny, Viacheslav O Nikolaev, Martin J Lohse, George G Holz, Michael W Roe.   

Abstract

Understanding the temporal and spatial integration of the Ca2+ and adenosine 3',5'-monophosphate (cAMP) signaling pathways requires concurrent measurements of both second messengers. Here, we describe an optical technique to simultaneously image cAMP and Ca2+ concentration gradients in MIN6 mouse insulinoma cells using Epac1-camps, a Förster (or fluorescence) resonance energy transfer (FRET)-based cAMP biosensor, and Fura-2, a fluorescent indicator of Ca2+. This real-time imaging method allows investigation of the dynamic organization and integration of multiple levels of signal processing in single living cells.

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Year:  2006        PMID： 16985238      PMCID： PMC3140640          DOI： 10.1126/stke.3532006pl6

Source DB:  PubMed          Journal:  Sci STKE        ISSN： 1525-8882


  15 in total

1.  Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin.

Authors:  A Miyawaki; J Llopis; R Heim; J M McCaffery; J A Adams; M Ikura; R Y Tsien
Journal:  Nature       Date:  1997-08-28       Impact factor: 49.962

2.  Novel single chain cAMP sensors for receptor-induced signal propagation.

Authors:  Viacheslav O Nikolaev; Moritz Bünemann; Lutz Hein; Annette Hannawacker; Martin J Lohse
Journal:  J Biol Chem       Date:  2004-07-01       Impact factor: 5.157

3.  Interplay of Ca2+ and cAMP signaling in the insulin-secreting MIN6 beta-cell line.

Authors:  Luis R Landa; Mark Harbeck; Kelly Kaihara; Oleg Chepurny; Kajorn Kitiphongspattana; Oliver Graf; Viacheslav O Nikolaev; Martin J Lohse; George G Holz; Michael W Roe
Journal:  J Biol Chem       Date:  2005-06-29       Impact factor: 5.157

4.  Fluorescence resonance energy transfer-based assays for the real-time detection of nitric oxide signaling.

Authors:  Claudette M St Croix; Molly S Stitt; Simon C Watkins; Bruce R Pitt
Journal:  Methods Enzymol       Date:  2005       Impact factor: 1.600

5.  A new generation of Ca2+ indicators with greatly improved fluorescence properties.

Authors:  G Grynkiewicz; M Poenie; R Y Tsien
Journal:  J Biol Chem       Date:  1985-03-25       Impact factor: 5.157

6.  Simultaneous measurements of intracellular cAMP and L-type Ca2+ current in single frog ventricular myocytes.

Authors:  J M Goaillard; P V Vincent; R Fischmeister
Journal:  J Physiol       Date:  2001-01-01       Impact factor: 5.182

7.  Biosensors to measure inositol 1,4,5-trisphosphate concentration in living cells with spatiotemporal resolution.

Authors:  Timothy P Remus; Aleksey V Zima; Julie Bossuyt; Dan J Bare; Jody L Martin; Lothar A Blatter; Donald M Bers; Gregory A Mignery
Journal:  J Biol Chem       Date:  2005-10-24       Impact factor: 5.157

8.  Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor.

Authors:  Amy E Palmer; Can Jin; John C Reed; Roger Y Tsien
Journal:  Proc Natl Acad Sci U S A       Date:  2004-12-07       Impact factor: 11.205

9.  Single cell Ca2+/cAMP cross-talk monitored by simultaneous Ca2+/cAMP fluorescence ratio imaging.

Authors:  M A DeBernardi; G Brooker
Journal:  Proc Natl Acad Sci U S A       Date:  1996-05-14       Impact factor: 11.205

10.  Termination of cAMP signals by Ca2+ and G(alpha)i via extracellular Ca2+ sensors: a link to intracellular Ca2+ oscillations.

Authors:  Andrea Gerbino; Warren C Ruder; Silvana Curci; Tullio Pozzan; Manuela Zaccolo; Aldebaran M Hofer
Journal:  J Cell Biol       Date:  2005-10-24       Impact factor: 10.539
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  21 in total

1.  Monomethylated-adenines potentiate glucose-induced insulin production and secretion via inhibition of phosphodiesterase activity in rat pancreatic islets.

Authors:  Brandon B Boland; Cristina Alarcón; Almas Ali; Christopher J Rhodes
Journal:  Islets       Date:  2015-09-24       Impact factor: 2.694

Review 2.  Reporting from the field: genetically encoded fluorescent reporters uncover signaling dynamics in living biological systems.

Authors:  Sohum Mehta; Jin Zhang
Journal:  Annu Rev Biochem       Date:  2011       Impact factor: 23.643

3.  Synchronizing Ca2+ and cAMP oscillations in pancreatic beta-cells: a role for glucose metabolism and GLP-1 receptors? Focus on "regulation of cAMP dynamics by Ca2+ and G protein-coupled receptors in the pancreatic beta-cell: a computational approach".

Authors:  George G Holz; Emma Heart; Colin A Leech
Journal:  Am J Physiol Cell Physiol       Date:  2007-11-07       Impact factor: 4.249

Review 4.  Kinetics of G-protein-coupled receptor signals in intact cells.

Authors:  M J Lohse; P Hein; C Hoffmann; V O Nikolaev; J-P Vilardaga; M Bünemann
Journal:  Br J Pharmacol       Date:  2008-01-14       Impact factor: 8.739

Review 5.  Glucagon-Like Peptide-1 and Its Class B G Protein-Coupled Receptors: A Long March to Therapeutic Successes.

Authors:  Chris de Graaf; Dan Donnelly; Denise Wootten; Jesper Lau; Patrick M Sexton; Laurence J Miller; Jung-Mo Ahn; Jiayu Liao; Madeleine M Fletcher; Dehua Yang; Alastair J H Brown; Caihong Zhou; Jiejie Deng; Ming-Wei Wang
Journal:  Pharmacol Rev       Date:  2016-10       Impact factor: 25.468

Review 6.  Imaging the coordination of multiple signalling activities in living cells.

Authors:  Christopher M Welch; Hunter Elliott; Gaudenz Danuser; Klaus M Hahn
Journal:  Nat Rev Mol Cell Biol       Date:  2011-10-21       Impact factor: 94.444

Review 7.  Imaging second messenger dynamics in developing neural circuits.

Authors:  Timothy A Dunn; Marla B Feller
Journal:  Dev Neurobiol       Date:  2008-05       Impact factor: 3.964

Review 8.  The ins and outs of adrenergic signaling.

Authors:  Martin J Lohse
Journal:  J Mol Med (Berl)       Date:  2015-07-23       Impact factor: 4.599

9.  Serum- and glucocorticoid-induced protein kinase 1 (SGK1) is regulated by store-operated Ca2+ entry and mediates cytoprotection against necrotic cell death.

Authors:  Deanna R Brickley; Abena S Agyeman; Richard F Kopp; Ben A Hall; Mark C Harbeck; Larissa Belova; Paul A Volden; Wei Wu; Michael W Roe; Suzanne D Conzen
Journal:  J Biol Chem       Date:  2013-09-16       Impact factor: 5.157

10.  Calcium-dependent increases in protein kinase-A activity in mouse retinal ganglion cells are mediated by multiple adenylate cyclases.

Authors:  Timothy A Dunn; Daniel R Storm; Marla B Feller
Journal:  PLoS One       Date:  2009-11-17       Impact factor: 3.240
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