| Literature DB >> 16973172 |
Chantal Eijsink1, Michel G D Kester, Marry E I Franke, Kees L M C Franken, Mirjam H M Heemskerk, Frans H J Claas, Arend Mulder.
Abstract
The ability of tetrameric major histocompatibility complex (MHC) class I-peptide complexes (tetramers) to detect antigen-specific T lymphocyte responses has yielded significant information about the generation of in vivo immunity in numerous antigenic systems. Here we present a novel method for rapid validation of tetrameric HLA molecules based on the presence of allodeterminants. Human monoclonal antibodies (mAbs) recognizing polymorphic determinants on HLA class I were immobilized on polystyrene microparticles and used to probe the structural integrity of tetrameric HLA class I molecules by flow cytometry. A total of 22 tetramers, based on HLA-A1, A2, A3, A24, B7 and B8 were reactive with their counterpart mAbs, thus confirming their antigenic integrity. A positive outcome of this mAb test ensures that tetrameric HLA class I can be used with greater confidence in subsequent functional assays.Entities:
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Year: 2006 PMID： 16973172 DOI： 10.1016/j.jim.2006.07.020
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303