Literature DB >> 1696183

Recombinant 70-kD protein used for determination of autoantigenic epitopes recognized by anti-RNP sera.

U Nyman1, I Lundberg, E Hedfors, I Pettersson.   

Abstract

A panel of high- and low-titre anti-RNP-positive patient sera was tested for reactivity with human snRNP proteins. The U1 snRNP-specific 70-kD peptide was found to be the most prominent RNP antigen recognized by high-titre anti-RNP sera, mainly found in patients with mixed connective tissue disease (MCTD). The reactivity with the 70-kD protein was further analysed with recombinant fusion proteins containing different segments of the protein. One major and two minor antigenic regions were found. Most patient sera, both with high- and low-titre anti-RNP antibodies, only recognized the major region. The most aminoterminal region, showing partial sequence homology with a mouse retroviral p30 gag protein, contained an epitope that was recognized by one serum only.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 1696183      PMCID： PMC1535023          DOI： 10.1111/j.1365-2249.1990.tb05290.x

Source DB:  PubMed          Journal:  Clin Exp Immunol        ISSN： 0009-9104            Impact factor:   4.330


  25 in total

1.  1958 Revision of diagnostic criteria for rheumatoid arthritis.

Authors:  M W ROPES; G A BENNETT; S COBB; R JACOX; R A JESSAR
Journal:  Bull Rheum Dis       Date:  1958-12

2.  Enzyme-linked immunosorbent assay using isolated (U) small nuclear ribonucleoprotein polypeptides as antigens to investigate the clinical significance of autoantibodies to these polypeptides.

Authors:  Y Takeda; G S Wang; R J Wang; S K Anderson; I Pettersson; S Amaki; G C Sharp
Journal:  Clin Immunol Immunopathol       Date:  1989-02

3.  The human U1-70K snRNP protein: cDNA cloning, chromosomal localization, expression, alternative splicing and RNA-binding.

Authors:  R A Spritz; K Strunk; C S Surowy; S O Hoch; D E Barton; U Francke
Journal:  Nucleic Acids Res       Date:  1987-12-23       Impact factor: 16.971

4.  Epitope mapping with a recombinant human 68-kDa (U1) ribonucleoprotein antigen reveals heterogeneous autoantibody profiles in human autoimmune sera.

Authors:  H H Guldner; H J Netter; C Szostecki; H J Lakomek; H Will
Journal:  J Immunol       Date:  1988-07-15       Impact factor: 5.422

5.  Human autoantibody secreted by immortalized lymphocyte cell line against the 68K polypeptide of the U1 small nuclear ribonucleoprotein.

Authors:  J T Chen; Y Takeda; W E Vanderslice; G C Sharp; I Pettersson; A Rosén; H Wigzell; R J Wang
Journal:  Arthritis Rheum       Date:  1988-10

6.  The use of immunoblotting and immunoprecipitation of (U) small nuclear ribonucleoproteins in the analysis of sera of patients with mixed connective tissue disease and systemic lupus erythematosus. A cross-sectional, longitudinal study.

Authors:  I Pettersson; G Wang; E I Smith; H Wigzell; E Hedfors; J Horn; G C Sharp
Journal:  Arthritis Rheum       Date:  1986-08

7.  Characteristics of a soluble nuclear antigen precipitating with sera of patients with systemic lupus erythematosus.

Authors:  E M Tan; H G Kunkel
Journal:  J Immunol       Date:  1966-03       Impact factor: 5.422

8.  The 1982 revised criteria for the classification of systemic lupus erythematosus.

Authors:  E M Tan; A S Cohen; J F Fries; A T Masi; D J McShane; N F Rothfield; J G Schaller; N Talal; R J Winchester
Journal:  Arthritis Rheum       Date:  1982-11

9.  Comparison between 3 diagnostic criteria for mixed connective tissue disease. Study of 593 patients.

Authors:  D Alarcón-Segovia; M H Cardiel
Journal:  J Rheumatol       Date:  1989-03       Impact factor: 4.666

10.  Quantitation of anti-RNP and anti-Sm antibodies in MCTD and SLE patients by immunoblotting.

Authors:  W J Habets; D J de Rooij; M H Hoet; L B van de Putte; W J van Venrooij
Journal:  Clin Exp Immunol       Date:  1985-02       Impact factor: 4.330
View more
  10 in total

1.  Key residues revealed in a major conformational epitope of the U1-70K protein.

Authors:  E Welin Henriksson; M Wahren-Herlenius; I Lundberg; E Mellquist; I Pettersson
Journal:  Proc Natl Acad Sci U S A       Date:  1999-12-07       Impact factor: 11.205

Review 2.  Mapping of epitopes recognized by anti-(U1)RNP autoantibodies.

Authors:  H H Guldner
Journal:  Mol Biol Rep       Date:  1992-06       Impact factor: 2.316

3.  Enteric Nervous System: Neuropathic Gastrointestinal Motility.

Authors:  Jackie D Wood
Journal:  Dig Dis Sci       Date:  2016-07       Impact factor: 3.199

4.  Blotting patterns of IgG anti-(U1)RNP antibodies in mixed connective tissue disease.

Authors:  A Ghirardello; A Doria; P Vesco; E Vaccaro; C Bernardi; C Catani; U Fagiolo; P F Gambari
Journal:  Rheumatol Int       Date:  1996       Impact factor: 2.631

Review 5.  The U1-snRNP complex: structural properties relating to autoimmune pathogenesis in rheumatic diseases.

Authors:  Nicole H Kattah; Michael G Kattah; Paul J Utz
Journal:  Immunol Rev       Date:  2010-01       Impact factor: 12.988

6.  Mapping epitopes of U1-70K autoantibodies at single-amino acid resolution.

Authors:  David James Haddon; Justin Ansel Jarrell; Vivian K Diep; Hannah E Wand; Jordan V Price; Stephanie Tangsombatvisit; Grace M Credo; Sally Mackey; Cornelia L Dekker; Emily C Baechler; Chih Long Liu; Madoo Varma; Paul J Utz
Journal:  Autoimmunity       Date:  2015-08-31       Impact factor: 2.815

7.  IgG and IgM anti-snRNP reactivity in sequentially obtained serum samples from patients with connective tissue diseases.

Authors:  U Nyman; I Lundberg; E Hedfors; M Wahren; I Pettersson
Journal:  Ann Rheum Dis       Date:  1992-12       Impact factor: 19.103

8.  Early targets of nuclear RNP humoral autoimmunity in human systemic lupus erythematosus.

Authors:  Brian D Poole; Rebecca I Schneider; Joel M Guthridge; Cathy A Velte; Morris Reichlin; John B Harley; Judith A James
Journal:  Arthritis Rheum       Date:  2009-03

9.  Sequential autoantigenic determinants of the small nuclear ribonucleoprotein Sm D shared by human lupus autoantibodies and MRL lpr/lpr antibodies.

Authors:  J A James; M J Mamula; J B Harley
Journal:  Clin Exp Immunol       Date:  1994-12       Impact factor: 4.330

10.  Anti-enteric neuronal antibodies and the irritable bowel syndrome.

Authors:  Jackie D Wood; Sumei Liu; Douglas A Drossman; Yehuda Ringel; William E Whitehead
Journal:  J Neurogastroenterol Motil       Date:  2012-01-16       Impact factor: 4.924
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.