Literature DB >> 3884199

Quantitation of anti-RNP and anti-Sm antibodies in MCTD and SLE patients by immunoblotting.

W J Habets, D J de Rooij, M H Hoet, L B van de Putte, W J van Venrooij.   

Abstract

A quantitative immunoblotting assay (QIBA) for the determination of specific antibody titres in human autoimmune sera is described. In this assay, a total HeLa nuclear protein fraction, immobilized on nitrocellulose blot strips, was used as source of antigens and immunoreactive species of autoantibodies were quantitated by an enzyme linked second antibody procedure. Besides being more discriminative, QIBA appeared to be up to 500 times more sensitive than immunodiffusion or immunoelectrophoresis. In this study we used 21 sera from patients with SLE or MCTD for a quantitative analysis of their specific autoantibody content. Within this group, a very diverse spectrum of antibody populations was observed; anti-RNP sera appeared to contain, among others, high tired antibody versus 70K and 31K polypeptides while all (n = 6) anti-Sm sera recognized a 25kD protein doublet. In a follow-up study of two MCTD patients significant flares in specific antibody content could be observed.

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Year:  1985        PMID： 3884199      PMCID： PMC1577150     

Source DB:  PubMed          Journal:  Clin Exp Immunol        ISSN： 0009-9104            Impact factor:   4.330


  22 in total

1.  Identification of antibodies to nuclear acidic antigens by counterimmunoelectrophoresis.

Authors:  N Kurata; E M Tan
Journal:  Arthritis Rheum       Date:  1976 May-Jun

2.  Identification of a nuclear protein (Scl-70) as a unique target of human antinuclear antibodies in scleroderma.

Authors:  A S Douvas; M Achten; E M Tan
Journal:  J Biol Chem       Date:  1979-10-25       Impact factor: 5.157

3.  Identification of human Sm and (U1) RNP antigens by immunoblotting.

Authors:  H H Guldner; H J Lakomek; F A Bautz
Journal:  J Immunol Methods       Date:  1983-11-11       Impact factor: 2.303

4.  Antibodies to Sm and RNP. Prognosticators of disease involvement.

Authors:  E F Munves; P H Schur
Journal:  Arthritis Rheum       Date:  1983-07

5.  Mixed connective tissue disease--an apparently distinct rheumatic disease syndrome associated with a specific antibody to an extractable nuclear antigen (ENA).

Authors:  G C Sharp; W S Irvin; E M Tan; R G Gould; H R Holman
Journal:  Am J Med       Date:  1972-02       Impact factor: 4.965

6.  Association of antibodies to ribonucleoprotein and Sm antigens with mixed connective-tissue disease, systematic lupus erythematosus and other rheumatic diseases.

Authors:  G C Sharp; W S Irvin; C M May; H R Holman; F C McDuffie; E V Hess; F R Schmid
Journal:  N Engl J Med       Date:  1976-11-18       Impact factor: 91.245

7.  Antibodies from patients with mixed connective tissue disease react with heterogeneous nuclear ribonucleoprotein or ribonucleic acid (hnRNP/RNA) of the nuclear matrix.

Authors:  M J Fritzler; R Ali; E M Tan
Journal:  J Immunol       Date:  1984-03       Impact factor: 5.422

8.  Synthesis and assembly of human small nuclear ribonucleoproteins generated by cell-free translation.

Authors:  D E Fisher; G E Conner; W H Reeves; G Blobel; H G Kunkel
Journal:  Proc Natl Acad Sci U S A       Date:  1983-10       Impact factor: 11.205

9.  Polypeptide components of human small nuclear ribonucleoproteins.

Authors:  J C Wooley; L R Zukerberg; S Y Chung
Journal:  Proc Natl Acad Sci U S A       Date:  1983-09       Impact factor: 11.205

10.  Characterization of the SS-B (La) antigen in adenovirus-infected and uninfected HeLa cells.

Authors:  W J Habets; J H den Brok; A M Boerbooms; L B van de Putte; W J van Venrooij
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
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  40 in total

Review 1.  The use of laboratory tests in the diagnosis of SLE.

Authors:  W Egner
Journal:  J Clin Pathol       Date:  2000-06       Impact factor: 3.411

2.  Heterogeneity of RNP and Sm autoantigens in relation to the cell sources and the activated state of the cells.

Authors:  F Hiepe; K Yamamoto; A Takeuchi; E Apostoloff; T Miyamoto
Journal:  Rheumatol Int       Date:  1990       Impact factor: 2.631

3.  Characterization by human antibodies of two HeLa cell proteins which are related to Xenopus laevis transcription factor TFIIIA.

Authors:  S Lagaye; J P Barque; M le Maire; H Denis; C J Larsen
Journal:  Nucleic Acids Res       Date:  1988-03-25       Impact factor: 16.971

4.  Accuracy of semiquantitative immunoenzymatic methods in quantitation of anti-topoisomerase I (Scl-70) antibodies.

Authors:  D Villalta; N Bizzaro; S Platzgummer; A Antico; M Tampoia; L Camogliano; D Bassetti; M Pradella; A Piazza; F Manoni; R Tozzoli; E Tonutti
Journal:  Clin Rheumatol       Date:  2004-12-10       Impact factor: 2.980

Review 5.  Antinuclear antibodies to RNA-binding proteins in SLE.

Authors:  P J Maddison
Journal:  Rheumatol Int       Date:  1991       Impact factor: 2.631

6.  Use of recombinant RNP peptides 70K and A in an ELISA for measurement of antibodies in mixed connective tissue disease: a longitudinal follow up of 18 patients.

Authors:  D J de Rooij; W J Habets; L B van de Putte; M H Hoet; A L Verbeek; W J van Venrooij
Journal:  Ann Rheum Dis       Date:  1990-06       Impact factor: 19.103

7.  Clinical significance of anti-RNP and anti-Sm autoantibodies as determined by immunoblotting and immunoprecipitation in sera from patients with connective tissue diseases.

Authors:  B Combe; M Rucheton; H Graafland; V Lussiez; C Brunel; J Sany
Journal:  Clin Exp Immunol       Date:  1989-01       Impact factor: 4.330

8.  A novel epitope on the C-terminus of SmD1 is recognized by the majority of sera from patients with systemic lupus erythematosus.

Authors:  G Riemekasten; J Marell; G Trebeljahr; R Klein; G Hausdorf; T Häupl; J Schneider-Mergener; G R Burmester; F Hiepe
Journal:  J Clin Invest       Date:  1998-08-15       Impact factor: 14.808

9.  T cell reactivity against the SmD1(83-119) C terminal peptide in patients with systemic lupus erythematosus.

Authors:  G Riemekasten; C Weiss; S Schneider; A Thiel; A Bruns; F Schumann; S Bläss; G-R Burmester; F Hiepe
Journal:  Ann Rheum Dis       Date:  2002-09       Impact factor: 19.103

10.  Antibodies to Sm and SS-A demonstrated by enzyme immunoassay. Correlation to clinical manifestations and disease activity in patients with systemic lupus erythematosus.

Authors:  M Gripenberg; A M Teppo; C Friman
Journal:  Rheumatol Int       Date:  1991       Impact factor: 2.631
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