Literature DB >> 16941567

Improved protocol for chromatofocusing on the ProteomeLab PF2D.

Olivier Barré1, Marc Solioz.   

Abstract

Beckman-Coulter has recently introduced the ProteomeLab PF2D for 2-D liquid separation of protein samples. The system features separation in the first dimension by chromatofocusing, followed by RP chromatography in the second dimension, allowing the analysis of complex proteomics samples. When used by the standard protocol, reproducibility and column life times are limited, making the use of the instrument very costly. We here present an improved protocol for chromatofocusing, which enhances column life by at least fivefold.

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Year:  2006        PMID： 16941567     DOI： 10.1002/pmic.200600074

Source DB:  PubMed          Journal:  Proteomics        ISSN： 1615-9853            Impact factor:   3.984


  5 in total

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Journal:  Mol Cell Proteomics       Date:  2010-10-29       Impact factor: 5.911

2.  The utilization of Triton X-100 for enhanced two-dimensional liquid-phase proteomics.

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Journal:  Trop Med Health       Date:  2012-10-24

4.  Cytochrome b5 and cytokeratin 17 are biomarkers in bronchoalveolar fluid signifying onset of acute lung injury.

Authors:  Antoine Ménoret; Sanjeev Kumar; Anthony T Vella
Journal:  PLoS One       Date:  2012-07-06       Impact factor: 3.240

5.  Multidimensional protein fractionation using ProteomeLab PF 2D for profiling amyotrophic lateral sclerosis immunity: A preliminary report.

Authors:  Joshua D Schlautman; Wojciech Rozek; Robert Stetler; R Lee Mosley; Howard E Gendelman; Pawel Ciborowski
Journal:  Proteome Sci       Date:  2008-09-12       Impact factor: 2.480
  5 in total

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