Literature DB >> 16928762

ORF3 protein of hepatitis E virus is not required for replication, virion assembly, or infection of hepatoma cells in vitro.

Suzanne U Emerson1, Hanh Nguyen, Udana Torian, Robert H Purcell.   

Abstract

A subclone of Huh-7 cells that could be relatively efficiently transfected and infected with hepatitis E virus was identified. Following transfection, infectious virus was produced but remained predominantly cell associated. Intracellular virus, recovered by lysis of transfected cells, infected naïve cells. This in vitro-produced virus appeared to be antigenically identical to virus isolated from clinical samples. Lysates from cells transfected with mutant viral genomes unable to synthesize ORF3 protein contained infectious virions that were similar in number, thermostability, and sedimentation characteristics to those in lysates transfected with wild-type viral genomes. Therefore, in contrast to its requirement in vivo, ORF3 protein is not required for infection of Huh-7 cells or production of infectious virus in vitro.

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Year:  2006        PMID： 16928762      PMCID： PMC1641774          DOI： 10.1128/JVI.00892-06

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  21 in total

1.  Thermal stability of hepatitis E virus.

Authors:  Suzanne U Emerson; Vidya A Arankalle; Robert H Purcell
Journal:  J Infect Dis       Date:  2005-07-12       Impact factor: 5.226

2.  A bicistronic subgenomic mRNA encodes both the ORF2 and ORF3 proteins of hepatitis E virus.

Authors:  Judith Graff; Udana Torian; Hanh Nguyen; Suzanne U Emerson
Journal:  J Virol       Date:  2006-06       Impact factor: 5.103

3.  Putative neutralization epitopes and broad cross-genotype neutralization of Hepatitis E virus confirmed by a quantitative cell-culture assay.

Authors:  Suzanne U Emerson; Pilar Clemente-Casares; Nasser Moiduddin; Vidya A Arankalle; Udana Torian; Robert H Purcell
Journal:  J Gen Virol       Date:  2006-03       Impact factor: 3.891

4.  Identification by phage display and characterization of two neutralizing chimpanzee monoclonal antibodies to the hepatitis E virus capsid protein.

Authors:  D J Schofield; J Glamann; S U Emerson; R H Purcell
Journal:  J Virol       Date:  2000-06       Impact factor: 5.103

5.  Virus-specific mRNA capping enzyme encoded by hepatitis E virus.

Authors:  J Magden; N Takeda; T Li; P Auvinen; T Ahola; T Miyamura; A Merits; L Kääriäinen
Journal:  J Virol       Date:  2001-07       Impact factor: 5.103

6.  Capped RNA transcripts of full-length cDNA clones of swine hepatitis E virus are replication competent when transfected into Huh7 cells and infectious when intrahepatically inoculated into pigs.

Authors:  Y W Huang; G Haqshenas; C Kasorndorkbua; P G Halbur; S U Emerson; X J Meng
Journal:  J Virol       Date:  2005-02       Impact factor: 5.103

7.  The ORF3 protein of hepatitis E virus is a phosphoprotein that associates with the cytoskeleton.

Authors:  M Zafrullah; M H Ozdener; S K Panda; S Jameel
Journal:  J Virol       Date:  1997-12       Impact factor: 5.103

8.  In vitro infection and replication of hepatitis E virus in primary cynomolgus macaque hepatocytes.

Authors:  A W Tam; R White; P O Yarbough; B J Murphy; C P McAtee; R E Lanford; T R Fuerst
Journal:  Virology       Date:  1997-11-10       Impact factor: 3.616

9.  Only the non-glycosylated fraction of hepatitis E virus capsid (open reading frame 2) protein is stable in mammalian cells.

Authors:  J Torresi; F Li; S A Locarnini; D A Anderson
Journal:  J Gen Virol       Date:  1999-05       Impact factor: 3.891

10.  The open reading frame 3 gene of hepatitis E virus contains a cis-reactive element and encodes a protein required for infection of macaques.

Authors:  Judith Graff; Hanh Nguyen; Claro Yu; William R Elkins; Marisa St Claire; Robert H Purcell; Suzanne U Emerson
Journal:  J Virol       Date:  2005-06       Impact factor: 5.103
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  59 in total

1.  Initiation at the third in-frame AUG codon of open reading frame 3 of the hepatitis E virus is essential for viral infectivity in vivo.

Authors:  Y W Huang; T Opriessnig; P G Halbur; X J Meng
Journal:  J Virol       Date:  2007-01-03       Impact factor: 5.103

2.  Genotype 3 diversity and quantification of hepatitis E virus RNA.

Authors:  Florence Abravanel; Karine Sandres-Saune; Sebastien Lhomme; Martine Dubois; Jean-Michel Mansuy; Jacques Izopet
Journal:  J Clin Microbiol       Date:  2012-01-11       Impact factor: 5.948

3.  Enhancement of interferon induction by ORF3 product of hepatitis E virus.

Authors:  Yuchen Nan; Zexu Ma; Rong Wang; Ying Yu; Harilakshmi Kannan; Brenda Fredericksen; Yan-Jin Zhang
Journal:  J Virol       Date:  2014-05-21       Impact factor: 5.103

4.  Hepatitis E virus ORF3 is a functional ion channel required for release of infectious particles.

Authors:  Qiang Ding; Brigitte Heller; Juan M V Capuccino; Bokai Song; Ila Nimgaonkar; Gabriela Hrebikova; Jorge E Contreras; Alexander Ploss
Journal:  Proc Natl Acad Sci U S A       Date:  2017-01-17       Impact factor: 11.205

5.  The PSAP motif within the ORF3 protein of an avian strain of the hepatitis E virus is not critical for viral infectivity in vivo but plays a role in virus release.

Authors:  Scott P Kenney; R S Pudupakam; Yao-Wei Huang; F William Pierson; Tanya LeRoith; Xiang-Jin Meng
Journal:  J Virol       Date:  2012-03-21       Impact factor: 5.103

6.  Hepatitis E virus replication requires an active ubiquitin-proteasome system.

Authors:  Yogesh A Karpe; Xiang-Jin Meng
Journal:  J Virol       Date:  2012-03-21       Impact factor: 5.103

7.  Dynamics of 8G12 competitive antibody in "prime-boost" vaccination of Hepatitis E vaccine.

Authors:  Xing Wu; Pan Chen; Huijuan Lin; Yao Su; Xiaotian Hao; Yufeng Cao; Li Li; Fengcai Zhu; Zhenglun Liang
Journal:  Hum Vaccin Immunother       Date:  2017-03-08       Impact factor: 3.452

8.  Development and validation of a negative-strand-specific reverse transcription-PCR assay for detection of a chicken strain of hepatitis E virus: identification of nonliver replication sites.

Authors:  P Billam; F W Pierson; W Li; T LeRoith; R B Duncan; X J Meng
Journal:  J Clin Microbiol       Date:  2008-06-18       Impact factor: 5.948

9.  The hepatitis E virus Orf3 protein protects cells from mitochondrial depolarization and death.

Authors:  Syed Mohammad Moin; Milena Panteva; Shahid Jameel
Journal:  J Biol Chem       Date:  2007-05-08       Impact factor: 5.157

10.  Hepatitis E virus genotype 1 infection of swine kidney cells in vitro is inhibited at multiple levels.

Authors:  H T Nguyen; P Shukla; U Torian; K Faulk; S U Emerson
Journal:  J Virol       Date:  2013-11-06       Impact factor: 5.103
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