Literature DB >> 1691181

Differential inhibition of transforming growth factor beta 1 and beta 2 activity by alpha 2-macroglobulin.

D Danielpour1, M B Sporn.   

Abstract

Affinity labeling and immunoprecipitation studies demonstrate that alpha 2-macroglobulin (alpha 2M) is the major serum-binding protein for transforming growth factors beta 1 and beta 2 (TGF-beta 1 and TGF-beta 2). Purified alpha 2M inhibits the binding of both 125I-TGF-beta 1 and 125I-TGF-beta 2 to cell surface receptors at I50 values of 200 and 10 micrograms/ml, respectively. alpha 2M (200 micrograms/ml) does not block TGF-beta 1 inhibition of CCL-64 mink lung cell growth but reduces this activity of TGF-beta 2 10-fold. The electrophoretic migration of 125I-TGF-beta.alpha 2M complexes on polyacrylamide gels under nondenaturing conditions demonstrates that alpha 2M has 10-fold greater affinity for TGF-beta 2 than for TGF-beta 1. Each of these complexes comigrates as a single band with the fast form of alpha 2M. We suggest that alpha 2M is an important differential regulator of the biological activities of TGF-beta 1 and TGF-beta 2 in vivo.

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Year:  1990        PMID： 1691181

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  29 in total

Review 1.  The extracellular regulation of growth factor action.

Authors:  R Flaumenhaft; D B Rifkin
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2.  Identification of a structural domain that distinguishes the actions of the type 1 and 2 isoforms of transforming growth factor beta on endothelial cells.

Authors:  S W Qian; J K Burmester; J R Merwin; J A Madri; M B Sporn; A B Roberts
Journal:  Proc Natl Acad Sci U S A       Date:  1992-07-15       Impact factor: 11.205

3.  Gene regulation networks related to neural differentiation of hESC.

Authors:  Jiang F Zhong; Yahui Song; Jing Du; Christine Gamache; Kathleen A Burke; Brett T Lund; Leslie P Weiner
Journal:  Gene Expr       Date:  2007

4.  Heterogeneity in the plasma levels of two acute-phase proteins in mice from inbred strains infected with Trypanosoma cruzi.

Authors:  M R Luz; F van Leuven; T C Araújo-Jorge
Journal:  Parasitol Res       Date:  1994       Impact factor: 2.289

5.  Neovascularization in aged mice: delayed angiogenesis is coincident with decreased levels of transforming growth factor beta1 and type I collagen.

Authors:  M J Reed; A Corsa; W Pendergrass; P Penn; E H Sage; I B Abrass
Journal:  Am J Pathol       Date:  1998-01       Impact factor: 4.307

6.  Vascular cells respond differentially to transforming growth factors beta 1 and beta 2 in vitro.

Authors:  J R Merwin; W Newman; L D Beall; A Tucker; J Madri
Journal:  Am J Pathol       Date:  1991-01       Impact factor: 4.307

7.  Low-affinity receptors for tumour necrosis factor-alpha, interferon-gamma and granulocyte-macrophage colony-stimulating factor are expressed on human placental syncytiotrophoblast.

Authors:  J Hampson; P J McLaughlin; P M Johnson
Journal:  Immunology       Date:  1993-07       Impact factor: 7.397

8.  Transforming growth factor beta 1 gene expression in human airways.

Authors:  J D Aubert; B I Dalal; T R Bai; C R Roberts; S Hayashi; J C Hogg
Journal:  Thorax       Date:  1994-03       Impact factor: 9.139

9.  Transforming growth factor beta upregulates 5-lipoxygenase activity during myeloid cell maturation.

Authors:  D Steinhilber; O Rådmark; B Samuelsson
Journal:  Proc Natl Acad Sci U S A       Date:  1993-07-01       Impact factor: 11.205

10.  Characterization of distinct functional domains of transforming growth factor beta.

Authors:  J K Burmester; S W Qian; A B Roberts; A Huang; S Amatayakul-Chantler; L Suardet; N Odartchenko; J A Madri; M B Sporn
Journal:  Proc Natl Acad Sci U S A       Date:  1993-09-15       Impact factor: 11.205
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