Literature DB >> 1631120

Identification of a structural domain that distinguishes the actions of the type 1 and 2 isoforms of transforming growth factor beta on endothelial cells.

S W Qian1, J K Burmester, J R Merwin, J A Madri, M B Sporn, A B Roberts.   

Abstract

A chimeric transforming growth factor beta (TGF-beta) molecule has been synthesized to map the amino acids responsible for the substantially greater activity of TGF-beta 1 than TGF-beta 2 on growth and migration of endothelial cells. This chimera consists of a dimer of a monomeric unit composed of amino acids 1-39 of TGF-beta 2, 40-82 of TGF-beta 1, and 83-112 of TGF-beta 2. Structural identity of the purified recombinant protein has been confirmed by immunoblotting and NH2-terminal sequencing. The biological potency of the TGF-beta 2-1-2 chimera was equal to that of TGF-beta 1 in inhibition of growth of both fetal bovine heart endothelial cells and rat epididymal fat pad microvascular endothelial cells. Similarly, the TGF-beta 2-1-2 chimera was nearly equivalent to TGF-beta 1 and at least 10-fold more active than TGF-beta 2 in inhibiting migration of bovine aortic endothelial cells. These results identify the sequence between amino acids 40-82 as an important region within TGF-beta that functions to specify a TGF-beta 1- or TGF-beta 2-like activity.

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Year:  1992        PMID： 1631120      PMCID： PMC49486          DOI： 10.1073/pnas.89.14.6290

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  33 in total

1.  Mesoderm induction in Xenopus laevis distinguishes between the various TGF-beta isoforms.

Authors:  A B Roberts; P Kondaiah; F Rosa; S Watanabe; P Good; D Danielpour; N S Roche; M L Rebbert; I B Dawid; M B Sporn
Journal:  Growth Factors       Date:  1990       Impact factor: 2.511

Review 2.  TGF-beta: problems and prospects.

Authors:  M B Sporn; A B Roberts
Journal:  Cell Regul       Date:  1990-11

3.  Identification of three amino acids in the platelet-derived growth factor (PDGF) B-chain that are important for binding to the PDGF beta-receptor.

Authors:  A Ostman; M Andersson; U Hellman; C H Heldin
Journal:  J Biol Chem       Date:  1991-06-05       Impact factor: 5.157

Review 4.  The transforming growth factor-beta family.

Authors:  J Massagué
Journal:  Annu Rev Cell Biol       Date:  1990

5.  Distinct transforming growth factor-beta (TGF-beta) receptor subsets as determinants of cellular responsiveness to three TGF-beta isoforms.

Authors:  S Cheifetz; H Hernandez; M Laiho; P ten Dijke; K K Iwata; J Massagué
Journal:  J Biol Chem       Date:  1990-11-25       Impact factor: 5.157

6.  Vascular cells respond differentially to transforming growth factors beta 1 and beta 2 in vitro.

Authors:  J R Merwin; W Newman; L D Beall; A Tucker; J Madri
Journal:  Am J Pathol       Date:  1991-01       Impact factor: 4.307

7.  Isoform-specific transforming growth factor-beta binding proteins with membrane attachments sensitive to phosphatidylinositol-specific phospholipase C.

Authors:  S Cheifetz; J Massagué
Journal:  J Biol Chem       Date:  1991-11-05       Impact factor: 5.157

8.  Two forms of transforming growth factor-beta distinguished by multipotential haematopoietic progenitor cells.

Authors:  M Ohta; J S Greenberger; P Anklesaria; A Bassols; J Massagué
Journal:  Nature       Date:  1987 Oct 8-14       Impact factor: 49.962

9.  Subtypes of betaglycan and of type I and type II transforming growth factor-beta (TGF-beta) receptors with different affinities for TGF-beta 1 and TGF-beta 2 are exhibited by human placental trophoblast cells.

Authors:  E J Mitchell; L Fitz-Gibbon; M D O'Connor-McCourt
Journal:  J Cell Physiol       Date:  1992-02       Impact factor: 6.384

10.  Identification and analysis of discrete functional domains in the pro region of pre-pro-transforming growth factor beta 1.

Authors:  X Sha; L Yang; L E Gentry
Journal:  J Cell Biol       Date:  1991-08       Impact factor: 10.539
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  7 in total

1.  Complex flexibility of the transforming growth factor beta superfamily.

Authors:  G Venkataraman; V Sasisekharan; C L Cooney; R Langer; R Sasisekharan
Journal:  Proc Natl Acad Sci U S A       Date:  1995-06-06       Impact factor: 11.205

2.  Characterization of transforming growth factor-beta (TGF-beta) receptors on BeWo choriocarcinoma cells including the identification of a novel 38-kDa TGF-beta binding glycoprotein.

Authors:  E J Mitchell; K Lee; M D O'Connor-McCourt
Journal:  Mol Biol Cell       Date:  1992-11       Impact factor: 4.138

3.  Molecular lesions associated with alleles of decapentaplegic identify residues necessary for TGF-beta/BMP cell signaling in Drosophila melanogaster.

Authors:  K Wharton; R P Ray; S D Findley; H E Duncan; W M Gelbart
Journal:  Genetics       Date:  1996-02       Impact factor: 4.562

4.  Characterization of distinct functional domains of transforming growth factor beta.

Authors:  J K Burmester; S W Qian; A B Roberts; A Huang; S Amatayakul-Chantler; L Suardet; N Odartchenko; J A Madri; M B Sporn
Journal:  Proc Natl Acad Sci U S A       Date:  1993-09-15       Impact factor: 11.205

5.  Role of transforming growth factor-beta in maintenance of function of cultured neonatal cardiac myocytes. Autocrine action and reversal of damaging effects of interleukin-1.

Authors:  A B Roberts; N S Roche; T S Winokur; J K Burmester; M B Sporn
Journal:  J Clin Invest       Date:  1992-11       Impact factor: 14.808

Review 6.  Transforming growth factor-beta: recent progress and new challenges.

Authors:  M B Sporn; A B Roberts
Journal:  J Cell Biol       Date:  1992-12       Impact factor: 10.539

7.  Biological activity differences between TGF-β1 and TGF-β3 correlate with differences in the rigidity and arrangement of their component monomers.

Authors:  Tao Huang; Seth L Schor; Andrew P Hinck
Journal:  Biochemistry       Date:  2014-09-05       Impact factor: 3.162
  7 in total

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