AIMS: To evaluate the presence of Shiga toxin-producing strains of Escherichia coli (STEC) of the O157:H7 serotype in living layer hens so as to analyse the role of this avian species as potential reservoir. METHODS AND RESULTS: Cloacal swabs were collected between November 2004 and November 2005 from four intensive management layer hen farms and analysed for STEC O157:H7 by immunomagnetic separation methods and multiplex polymerase chain reaction for stx1 and/or stx2, the E. coli attaching and effacing (eae) and hly genes. STEC was detected in 26 of the 720 samples. CONCLUSIONS: The layer hens analysed were shown to carry STEC O157:H7. The presence of this bacterium in living layer hen farms investigated did not result in any detectable increase in gastrointestinal disease in this species. SIGNIFICANCE AND IMPACT OF THE STUDY: Living layer hens are a novel potential reservoir of E. coli O157:H7.
AIMS: To evaluate the presence of Shiga toxin-producing strains of Escherichia coli (STEC) of the O157:H7 serotype in living layer hens so as to analyse the role of this avian species as potential reservoir. METHODS AND RESULTS: Cloacal swabs were collected between November 2004 and November 2005 from four intensive management layer hen farms and analysed for STEC O157:H7 by immunomagnetic separation methods and multiplex polymerase chain reaction for stx1 and/or stx2, the E. coli attaching and effacing (eae) and hly genes. STEC was detected in 26 of the 720 samples. CONCLUSIONS: The layer hens analysed were shown to carry STEC O157:H7. The presence of this bacterium in living layer hen farms investigated did not result in any detectable increase in gastrointestinal disease in this species. SIGNIFICANCE AND IMPACT OF THE STUDY: Living layer hens are a novel potential reservoir of E. coli O157:H7.
Authors: O Pinaka; S Pournaras; V Mouchtouri; E Plakokefalos; A Katsiaflaka; F Kolokythopoulou; E Barboutsi; N Bitsolas; C Hadjichristodoulou Journal: Eur J Clin Microbiol Infect Dis Date: 2013-05-16 Impact factor: 3.267
Authors: S Baron; S Delannoy; S Bougeard; E Larvor; E Jouy; O Balan; P Fach; I Kempf Journal: Antimicrob Agents Chemother Date: 2015-12-14 Impact factor: 5.191
Authors: Safaa Abd El-Moneim Mohammed; Sherif Abd El-Moneimb Marouf; Ahmed M Erfana; Jakeen Kamal Abd El-Haleem El-Jakee; Ashgan M Hessain; Turki M Dawoud; Saleh A Kabli; Ihab M Moussa Journal: Saudi J Biol Sci Date: 2018-07-20 Impact factor: 4.219