| Literature DB >> 16910642 |
In Su Lee1, Nohyun Lee, Jongnam Park, Byung Hyo Kim, Yong-Weon Yi, Taeuk Kim, Tae Kook Kim, In Hwan Lee, Seung R Paik, Taeghwan Hyeon.
Abstract
Ni/NiO core/shell nanoparticles having high affinity with polyhistidine were synthesized by decomposition of a Ni surfactant complex followed by air oxidation. Ni/NiO nanoparticles showed selective and efficient binding to histidine-tagged proteins and easy separation by using a magnet. These provided a more convenient way to efficient purification of histidine-tagged proteins compared with the conventional Ni-NTA complex-bound resins and microbeads.Entities:
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Year: 2006 PMID: 16910642 DOI: 10.1021/ja063177n
Source DB: PubMed Journal: J Am Chem Soc ISSN: 0002-7863 Impact factor: 15.419