Literature DB >> 16910642

Ni/NiO core/shell nanoparticles for selective binding and magnetic separation of histidine-tagged proteins.

In Su Lee1, Nohyun Lee, Jongnam Park, Byung Hyo Kim, Yong-Weon Yi, Taeuk Kim, Tae Kook Kim, In Hwan Lee, Seung R Paik, Taeghwan Hyeon.   

Abstract

Ni/NiO core/shell nanoparticles having high affinity with polyhistidine were synthesized by decomposition of a Ni surfactant complex followed by air oxidation. Ni/NiO nanoparticles showed selective and efficient binding to histidine-tagged proteins and easy separation by using a magnet. These provided a more convenient way to efficient purification of histidine-tagged proteins compared with the conventional Ni-NTA complex-bound resins and microbeads.

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Year:  2006        PMID: 16910642     DOI: 10.1021/ja063177n

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN: 0002-7863            Impact factor:   15.419


  30 in total

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