Literature DB >> 1689153

Nucleotide sequence of the raw-starch-digesting amylase gene from Bacillus sp. B1018 and its strong homology to the cyclodextrin glucanotransferase genes.

P Itkor1, N Tsukagoshi, S Udaka.   

Abstract

The gene coding for the raw-starch-digesting amylase from Bacillus sp. B1018 was cloned into Escherichia coli, and its nucleotide sequence was determined. Starting at an ATG codon, there was an open reading frame composed of 2139 bp (713 amino acids). The NH2-terminal portion encoded a 27 amino acid-long signal peptide. S1 mapping revealed the presence of a typical promoter region upstream from the transcription initiation site. The deduced amino acid sequence of the extracellular mature enzyme was very similar to those of cyclodextrin glucanotransferases (CGTases), especially that of alkalophilic Bacillus sp. 1011 (87% homology). In fact, the B1018 amylase showed CGTase activity. The COOH-terminal portion of the B1018 amylase shows significant homology with other raw-starch-digesting enzymes.

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Year:  1990        PMID： 1689153     DOI： 10.1016/0006-291x(90)90855-h

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  12 in total

1.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1990-08-11       Impact factor: 16.971

2.  Raw-starch-digesting and thermostable alpha-amylase from the yeast Cryptococcus sp. S-2: purification, characterization, cloning and sequencing.

Authors:  H Iefuji; M Chino; M Kato; Y Iimura
Journal:  Biochem J       Date:  1996-09-15       Impact factor: 3.857

3.  Identification of sigma S-regulated genes in Salmonella typhimurium: complementary regulatory interactions between sigma S and cyclic AMP receptor protein.

Authors:  F C Fang; C Y Chen; D G Guiney; Y Xu
Journal:  J Bacteriol       Date:  1996-09       Impact factor: 3.490

Review 4.  Remarkable evolutionary relatedness among the enzymes and proteins from the α-amylase family.

Authors:  Štefan Janeček; Marek Gabriško
Journal:  Cell Mol Life Sci       Date:  2016-05-06       Impact factor: 9.261

5.  Characteristics of two forms of alpha-amylases and structural implication.

Authors:  K Ohdan; T Kuriki; H Kaneko; J Shimada; T Takada; Z Fujimoto; H Mizuno; S Okada
Journal:  Appl Environ Microbiol       Date:  1999-10       Impact factor: 4.792

6.  Characteristic differences in the primary structure allow discrimination of cyclodextrin glucanotransferases from alpha-amylases.

Authors:  S Janecek; E A MacGregor; B Svensson
Journal:  Biochem J       Date:  1995-01-15       Impact factor: 3.857

7.  Trehalose synthesis by sequential reactions of recombinant maltooligosyltrehalose synthase and maltooligosyltrehalose trehalohydrolase from Brevibacterium helvolum.

Authors:  Y H Kim; T K Kwon; S Park; H S Seo; J J Cheong; C H Kim; J K Kim; J S Lee; Y D Choi
Journal:  Appl Environ Microbiol       Date:  2000-11       Impact factor: 4.792

8.  Cloning of the beta-amylase gene from Bacillus cereus and characteristics of the primary structure of the enzyme.

Authors:  T Nanmori; M Nagai; Y Shimizu; R Shinke; B Mikami
Journal:  Appl Environ Microbiol       Date:  1993-02       Impact factor: 4.792

9.  Cyclodextrin formation by the thermostable alpha-amylase of Thermoanaerobacterium thermosulfurigenes EM1 and reclassification of the enzyme as a cyclodextrin glycosyltransferase.

Authors:  R D Wind; W Liebl; R M Buitelaar; D Penninga; A Spreinat; L Dijkhuizen; H Bahl
Journal:  Appl Environ Microbiol       Date:  1995-04       Impact factor: 4.792

Review 10.  Engineering of cyclodextrin glucanotransferases and the impact for biotechnological applications.

Authors:  Hans Leemhuis; Ronan M Kelly; Lubbert Dijkhuizen
Journal:  Appl Microbiol Biotechnol       Date:  2009-09-18       Impact factor: 4.813
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