Literature DB >> 1688839

Detection of lipopolysaccharides by ethidium bromide staining after sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

N Kido1, M Ohta, N Kato.   

Abstract

A rapid and easy method for staining lipopolysaccharides with ethidium bromide is described. Lipopolysaccharides could be visualized by ethidium bromide with almost the same sensitivity as found with the silver-staining method in less than 30 min. The ethidium bromide-staining method was particularly suitable for staining lipopolysaccharides possessing acidic O-specific polysaccharides, which were poorly visualized by silver staining.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 1688839      PMCID： PMC208550          DOI： 10.1128/jb.172.2.1145-1147.1990

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  12 in total

1.  The identification of 2-amino-2-deoxy-L-altruronic acid as a constituent of Shigella sonnei phase I lipopolysaccharide.

Authors:  T Kontrohr
Journal:  Carbohydr Res       Date:  1977-10       Impact factor: 2.104

2.  The O8 antigen of Escherichia coli. Structure of the polysaccharide chain.

Authors:  K Reske; K Jann
Journal:  Eur J Biochem       Date:  1972-12-04

3.  Heterogeneity of antigenic-side-chain length in lipopolysaccharide from Escherichia coli 0111 and Salmonella typhimurium LT2.

Authors:  R C Goldman; L Leive
Journal:  Eur J Biochem       Date:  1980

4.  Heterogeneity of lipopolysaccharides. Analysis of polysaccharide chain lengths by sodium dodecylsulfate-polyacrylamide gel electrophoresis.

Authors:  B Jann; K Reske; K Jann
Journal:  Eur J Biochem       Date:  1975-12-01

5.  A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels.

Authors:  C M Tsai; C E Frasch
Journal:  Anal Biochem       Date:  1982-01-01       Impact factor: 3.365

6.  Somatic antigen of Shigella dysenteriae type 3. Structural features of specific polysaccharide chain.

Authors:  B A Dmitriev; L V Backinowsky; V L Lvov; N K Kochetkov; I L Hofman
Journal:  Eur J Biochem       Date:  1975-01-15

7.  Partial deletion of the cloned rfb gene of Escherichia coli O9 results in synthesis of a new O-antigenic lipopolysaccharide.

Authors:  N Kido; M Ohta; K Iida; T Hasegawa; H Ito; Y Arakawa; T Komatsu; N Kato
Journal:  J Bacteriol       Date:  1989-07       Impact factor: 3.490

8.  SDS-polyacrylamide gel electrophoresis of lipopolysaccharides.

Authors:  R R Russell; K G Johnson
Journal:  Can J Microbiol       Date:  1975-12       Impact factor: 2.419

9.  Size heterogeneity of Salmonella typhimurium lipopolysaccharides in outer membranes and culture supernatant membrane fragments.

Authors:  R S Munford; C L Hall; P D Rick
Journal:  J Bacteriol       Date:  1980-11       Impact factor: 3.490

10.  Somatic antigens of Shigella. The structure of the specific polysaccharide of Shigella newcastle (Sh. flexneri type 6) lipopolysaccharide.

Authors:  B A Dmitriev; Y A Knirel; O K Sheremet; A A Shashkov; N K Kochetkov; I L Hofman
Journal:  Eur J Biochem       Date:  1979-07
View more
  15 in total

1.  Involvement of Genes on a Megaplasmid in the Acid-Tolerant Phenotype of Rhizobium leguminosarum Biovar Trifolii.

Authors:  H Chen; E Gartner; B G Rolfe
Journal:  Appl Environ Microbiol       Date:  1993-04       Impact factor: 4.792

2.  Detection of two smooth colony phenotypes in a Salmonella enteritidis isolate which vary in their ability to contaminate eggs.

Authors:  J G Petter
Journal:  Appl Environ Microbiol       Date:  1993-09       Impact factor: 4.792

3.  Locating of nucleic acid intercalators in yeast cells by image analysis combined fluorescence microscopy.

Authors:  Evgeny O Puchkov; Millicent McCarren
Journal:  J Fluoresc       Date:  2010-12-03       Impact factor: 2.217

4.  Biochemical properties of the outer membrane of Treponema denticola.

Authors:  W W Yotis; V K Sharma; C Gopalsami; S Chegini; J McNulty; K Hoerman; J Keene; L G Simonson
Journal:  J Clin Microbiol       Date:  1991-07       Impact factor: 5.948

5.  Electrophoretic and chemical characterization of lipopolysaccharides of Vibrio parahaemolyticus.

Authors:  T J Han; T J Chai
Journal:  J Bacteriol       Date:  1992-05       Impact factor: 3.490

6.  Characterization of mutants of Caulobacter crescentus defective in surface attachment of the paracrystalline surface layer.

Authors:  S G Walker; D N Karunaratne; N Ravenscroft; J Smit
Journal:  J Bacteriol       Date:  1994-10       Impact factor: 3.490

7.  TnphoA Salmonella abortusovis mutants unable to adhere to epithelial cells and with reduced virulence in mice.

Authors:  S Rubino; G Leori; P Rizzu; G Erre; M M Colombo; S Uzzau; G Masala; P Cappuccinelli
Journal:  Infect Immun       Date:  1993-05       Impact factor: 3.441

8.  Expression of the cloned Escherichia coli O9 rfb gene in various mutant strains of Salmonella typhimurium.

Authors:  T Sugiyama; N Kido; T Komatsu; M Ohta; N Kato
Journal:  J Bacteriol       Date:  1991-01       Impact factor: 3.490

9.  Comparative study of biological properties and electrophoretic characteristics of lipopolysaccharide from eel-virulent and eel-A virulent Vibrio vulnificus strains.

Authors:  E G Biosca; R M Collado; J D Oliver; C Amaro
Journal:  Appl Environ Microbiol       Date:  1999-02       Impact factor: 4.792

10.  Electrophoretic analysis of heterogeneous lipopolysaccharides from various strains of Vibrio vulnificus biotypes 1 and 2 by silver staining and immunoblotting.

Authors:  C Amaro; E G Biosca; B Fouz; E Garay
Journal:  Curr Microbiol       Date:  1992-08       Impact factor: 2.188
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.