Literature DB >> 16880565

Purification, crystallization and preliminary crystallographic analysis of mouse myo-inositol oxygenase.

Peter M Brown1, Tom T Caradoc-Davies, James M Dickson, Garth J S Cooper, Kerry M Loomes, Edward N Baker.   

Abstract

Myo-inositol oxygenase (MIOX) catalyzes the novel oxidative cleavage of myo-inositol (MI) and its epimer D-chiro inositol (DCI) to D-glucuronate. MIOX utilizes an Fe(II)/Fe(III) binuclear iron centre for the dioxygen-dependent cleavage of the C1-C6 bond in MI. Despite its key role in inositol metabolism, the structural basis of its unique four-electron oxidation mechanism and its substrate specificity remain unknown. In order to answer these questions and to facilitate the use of this key enzyme for the development of new therapeutic strategies for diabetes, the mouse enzyme has been cloned, expressed in Escherichia coli, purified and crystallized from 4.4 M sodium formate. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 44.87, b = 77.26, c = 84.84 angstroms, and diffract to 2.8 angstroms resolution.

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Year:  2006        PMID： 16880565      PMCID： PMC2242914          DOI： 10.1107/S1744309106028144

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  12 in total

1.  Biochemistry and molecular cell biology of diabetic complications.

Authors:  M Brownlee
Journal:  Nature       Date:  2001-12-13       Impact factor: 49.962

2.  myo-Inositol oxygenase: molecular cloning and expression of a unique enzyme that oxidizes myo-inositol and D-chiro-inositol.

Authors:  R J Arner; K S Prabhu; J T Thompson; G R Hildenbrandt; A D Liken; C C Reddy
Journal:  Biochem J       Date:  2001-12-01       Impact factor: 3.857

3.  myo-Inositol oxygenase from hog kidney. II. Catalytic properties of the homogeneous enzyme.

Authors:  C C Reddy; P A Pierzchala; G A Hamilton
Journal:  J Biol Chem       Date:  1981-08-25       Impact factor: 5.157

4.  Modulation of renal-specific oxidoreductase/myo-inositol oxygenase by high-glucose ambience.

Authors:  Baibaswata Nayak; Ping Xie; Shigeru Akagi; Qiwei Yang; Lin Sun; Jun Wada; Arun Thakur; Farhad R Danesh; Sumant S Chugh; Yashpal S Kanwar
Journal:  Proc Natl Acad Sci U S A       Date:  2005-12-05       Impact factor: 11.205

5.  Expression of myo-inositol oxygenase in tissues susceptible to diabetic complications.

Authors:  Ryan J Arner; K Sandeep Prabhu; Venkatesh Krishnan; Mary C Johnson; C Channa Reddy
Journal:  Biochem Biophys Res Commun       Date:  2005-11-28       Impact factor: 3.575

6.  Identification of a renal-specific oxido-reductase in newborn diabetic mice.

Authors:  Q Yang; B Dixit; J Wada; Y Tian; E I Wallner; S K Srivastva; Y S Kanwar
Journal:  Proc Natl Acad Sci U S A       Date:  2000-08-29       Impact factor: 11.205

7.  Endothelins: regulators of extracellular matrix protein production in diabetes.

Authors:  Zia A Khan; Hana Farhangkhoee; Jeffery L Mahon; Lynda Bere; John R Gonder; Bosco M Chan; Shashi Uniyal; Subrata Chakrabarti
Journal:  Exp Biol Med (Maywood)       Date:  2006-06

8.  Chiroinositol deficiency and insulin resistance. II. Acute effects of D-chiroinositol administration in streptozotocin-diabetic rats, normal rats given a glucose load, and spontaneously insulin-resistant rhesus monkeys.

Authors:  H K Ortmeyer; L C Huang; L Zhang; B C Hansen; J Larner
Journal:  Endocrinology       Date:  1993-02       Impact factor: 4.736

Review 9.  The integration of macromolecular diffraction data.

Authors:  Andrew G W Leslie
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2005-12-14

Review 10.  Scaling and assessment of data quality.

Authors:  Philip Evans
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2005-12-14
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  7 in total

Review 1.  The "Other" Inositols and Their Phosphates: Synthesis, Biology, and Medicine (with Recent Advances in myo-Inositol Chemistry).

Authors:  Mark P Thomas; Stephen J Mills; Barry V L Potter
Journal:  Angew Chem Int Ed Engl       Date:  2015-12-22       Impact factor: 15.336

2.  Two duplicated genes DDI2 and DDI3 in budding yeast encode a cyanamide hydratase and are induced by cyanamide.

Authors:  Jia Li; Michael Biss; Yu Fu; Xin Xu; Stanley A Moore; Wei Xiao
Journal:  J Biol Chem       Date:  2015-04-06       Impact factor: 5.157

3.  Crystal structure of a substrate complex of myo-inositol oxygenase, a di-iron oxygenase with a key role in inositol metabolism.

Authors:  Peter M Brown; Tom T Caradoc-Davies; James M J Dickson; Garth J S Cooper; Kerry M Loomes; Edward N Baker
Journal:  Proc Natl Acad Sci U S A       Date:  2006-09-29       Impact factor: 11.205

4.  Common ancestry and novel genetic traits of Francisella novicida-like isolates from North America and Australia as revealed by comparative genomic analyses.

Authors:  Shivakumara Siddaramappa; Jean F Challacombe; Jeannine M Petersen; Segaran Pillai; Geoff Hogg; Cheryl R Kuske
Journal:  Appl Environ Microbiol       Date:  2011-06-10       Impact factor: 4.792

Review 5.  Structure/function correlations over binuclear non-heme iron active sites.

Authors:  Edward I Solomon; Kiyoung Park
Journal:  J Biol Inorg Chem       Date:  2016-07-01       Impact factor: 3.358

6.  Circular dichroism, magnetic circular dichroism, and variable temperature variable field magnetic circular dichroism studies of biferrous and mixed-valent myo-inositol oxygenase: insights into substrate activation of O2 reactivity.

Authors:  Rae Ana Snyder; Caleb B Bell; Yinghui Diao; Carsten Krebs; J Martin Bollinger; Edward I Solomon
Journal:  J Am Chem Soc       Date:  2013-10-15       Impact factor: 15.419

7.  Genetic diversity within the genus Francisella as revealed by comparative analyses of the genomes of two North American isolates from environmental sources.

Authors:  Shivakumara Siddaramappa; Jean F Challacombe; Jeannine M Petersen; Segaran Pillai; Cheryl R Kuske
Journal:  BMC Genomics       Date:  2012-08-24       Impact factor: 3.969
  7 in total

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