BACKGROUND: Total plasma homocysteine has emerged as an independent risk factor for vascular disease. To meet increasing requests by clinicians for this homocysteine determination, a rapid assay for a routine use has been developed. METHODS: A robust, stable, isotope-dilution liquid chromatography tandem mass spectrometry (LC/MS/MS) method is described, including all the practical details and analytical performance results. RESULTS: The method allows homocysteine quantitation over a linear working range up to 100 micromol/L, with the limit of quantification estimated at a low value of 0.09 micromol/L. Total analytical imprecision is less than 4%. Accuracy was assessed by measuring the homocysteine concentration in a serum Standard Reference Material. CONCLUSIONS: The method was demonstrated to be quick, reliable and cheap after 1 year of use on a time-shared instrument in our hospital unit.
BACKGROUND: Total plasma homocysteine has emerged as an independent risk factor for vascular disease. To meet increasing requests by clinicians for this homocysteine determination, a rapid assay for a routine use has been developed. METHODS: A robust, stable, isotope-dilution liquid chromatography tandem mass spectrometry (LC/MS/MS) method is described, including all the practical details and analytical performance results. RESULTS: The method allows homocysteine quantitation over a linear working range up to 100 micromol/L, with the limit of quantification estimated at a low value of 0.09 micromol/L. Total analytical imprecision is less than 4%. Accuracy was assessed by measuring the homocysteine concentration in a serum Standard Reference Material. CONCLUSIONS: The method was demonstrated to be quick, reliable and cheap after 1 year of use on a time-shared instrument in our hospital unit.
Authors: David Mischoulon; Jonathan E Alpert; Erland Arning; Teodoro Bottiglieri; Maurizio Fava; George I Papakostas Journal: J Clin Psychiatry Date: 2012-05-15 Impact factor: 4.384
Authors: Shao-Chiang Lai; Yasumi Nakayama; Jeffrey M Sequeira; Bogdan J Wlodarczyk; Robert M Cabrera; Richard H Finnell; Teodoro Bottiglieri; Edward V Quadros Journal: FASEB J Date: 2013-02-21 Impact factor: 5.191
Authors: M A H den Hoed; E Lopez-Lopez; M L te Winkel; W Tissing; J D E de Rooij; A Gutierrez-Camino; A Garcia-Orad; E den Boer; R Pieters; S M F Pluijm; R de Jonge; M M van den Heuvel-Eibrink Journal: Pharmacogenomics J Date: 2014-11-04 Impact factor: 3.550
Authors: Daniel S Lupu; Luz D Orozco; Ying Wang; John M Cullen; Matteo Pellegrini; Steven H Zeisel Journal: FASEB J Date: 2017-02-08 Impact factor: 5.191