José F Siqueira1, Isabela N Rôças. 1. Department of Endodontics, Estácio de Sá University, Rio de Janeiro, Brazil. siqueira@estacio.br
Abstract
OBJECTIVE: This study intended to examine samples from primary endodontic infections for the presence of Catonella morbi and Granulicatella adiacens, 2 species that have been recently suggested to be involved with infections in other oral sites. STUDY DESIGN: Genomic DNA was isolated directly from samples taken from teeth with different forms of apical periodontitis, and a devised culture-independent 16S rRNA gene-based heminested PCR assay was used to determine the prevalence of these 2 target species. Species-specific primers were developed by aligning closely related 16S rRNA gene sequences. Species-specificity for each primer pair was confirmed by running PCR against a panel of oral bacteria and by sequencing of DNA from representative positive samples. RESULTS: C morbi and G adiacens were detected in 33% and 19%, respectively, of the root canals associated with chronic apical periodontitis; 30% and 10%, respectively, of the cases diagnosed as acute apical periodontitis, and 16% and 11%, respectively, of the pus samples taken from acute apical abscesses. Overall, C morbi occurred in 26% and G adiacens in 14% of the samples taken from primary endodontic infections. CONCLUSIONS: Our findings demonstrate that C morbi and G adiacens can take part in the microbiota associated with primary endodontic infections, and their specific role in the disease process warrants further elucidation.
OBJECTIVE: This study intended to examine samples from primary endodontic infections for the presence of Catonella morbi and Granulicatella adiacens, 2 species that have been recently suggested to be involved with infections in other oral sites. STUDY DESIGN: Genomic DNA was isolated directly from samples taken from teeth with different forms of apical periodontitis, and a devised culture-independent 16S rRNA gene-based heminested PCR assay was used to determine the prevalence of these 2 target species. Species-specific primers were developed by aligning closely related 16S rRNA gene sequences. Species-specificity for each primer pair was confirmed by running PCR against a panel of oral bacteria and by sequencing of DNA from representative positive samples. RESULTS: C morbi and G adiacens were detected in 33% and 19%, respectively, of the root canals associated with chronic apical periodontitis; 30% and 10%, respectively, of the cases diagnosed as acute apical periodontitis, and 16% and 11%, respectively, of the pus samples taken from acute apical abscesses. Overall, C morbi occurred in 26% and G adiacens in 14% of the samples taken from primary endodontic infections. CONCLUSIONS: Our findings demonstrate that C morbi and G adiacens can take part in the microbiota associated with primary endodontic infections, and their specific role in the disease process warrants further elucidation.
Authors: James J Farrell; Lei Zhang; Hui Zhou; David Chia; David Elashoff; David Akin; Bruce J Paster; Kaumudi Joshipura; David T W Wong Journal: Gut Date: 2011-10-12 Impact factor: 23.059
Authors: Cheryl A Nickerson; C Mark Ott; Sarah L Castro; Veronica M Garcia; Thomas C Molina; Jeffrey T Briggler; Amber L Pitt; Joseph J Tavano; J Kelly Byram; Jennifer Barrila; Max A Nickerson Journal: PLoS One Date: 2011-12-19 Impact factor: 3.240
Authors: Bo Liu; Lina L Faller; Niels Klitgord; Varun Mazumdar; Mohammad Ghodsi; Daniel D Sommer; Theodore R Gibbons; Todd J Treangen; Yi-Chien Chang; Shan Li; O Colin Stine; Hatice Hasturk; Simon Kasif; Daniel Segrè; Mihai Pop; Salomon Amar Journal: PLoS One Date: 2012-06-04 Impact factor: 3.240