Effects of the neuropeptide APGW-amide and related compounds on molluscan muscles--GW-amide shows potent modulatory effects.

1. Effects of the molluscan neuropeptide APGW-amide and related compounds (the crustacean hormone RPCH, FAPGW-amide, PGW-amide, GW-amide and W-amide) were examined in several kinds of molluscan muscles. 2. All the compounds, except W-amide, showed qualitatively similar modulatory effects on contraction or relaxation of the muscles. The potency order of the compounds was found to be GW-amide greater than or equal to APGW-amide greater than FAPGW-amide greater than RPCH greater than PGW-amide. W-amide showed little or no effect even at 10(-4) M. 3. In the ABRM of Mytilus and the radula retractor muscle of Rapana, the active peptides were suggested to exhibit their modulatory effects by acting on the presynaptic sites in the muscles. 4. Nineteen GW-amide analogues, such as Gly-Trp-OH, Gly-Phe-NH2, D-Ala-Trp-NH2 and N-Gly-tryptamine, were also tested on some of the muscles, but all of them showed little or no effect.