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Literature DB >> 16872749

Digitisation of electron microscope films: six useful tests applied to three film scanners.

R Henderson¹, D Cattermole, G McMullan, S Scotcher, M Fordham, W B Amos, A R Faruqi.

Abstract

A series of simple tests have been used to measure the performance of flat-bed film scanners suitable for digitisation of electron micrographs. Two of the film scanners evaluated are commercially available and one has been constructed in the laboratory paying special attention to the needs of the electron microscopist. The tests may be useful for others.

Entities: Chemical

Mesh：

Year: 2006 PMID： 16872749 DOI： 10.1016/j.ultramic.2006.05.003

Source DB: PubMed Journal: Ultramicroscopy ISSN： 0304-3991 Impact factor: 2.689

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Cited

11 in total

1. Averaging tens to hundreds of icosahedral particle images to resolve protein secondary structure elements using a Multi-Path Simulated Annealing optimization algorithm.

Authors: Xiangan Liu; Wen Jiang; Joanita Jakana; Wah Chiu
Journal: J Struct Biol Date: 2007-07-06 Impact factor: 2.867

2. An algorithm for estimation and correction of anisotropic magnification distortion of cryo-EM images without need of pre-calibration.

Authors: Guimei Yu; Kunpeng Li; Yue Liu; Zhenguo Chen; Zhiqing Wang; Rui Yan; Thomas Klose; Liang Tang; Wen Jiang
Journal: J Struct Biol Date: 2016-06-04 Impact factor: 2.867

Digitisation of electron microscope films: six useful tests applied to three film scanners.

1. Averaging tens to hundreds of icosahedral particle images to resolve protein secondary structure elements using a Multi-Path Simulated Annealing optimization algorithm.

2. An algorithm for estimation and correction of anisotropic magnification distortion of cryo-EM images without need of pre-calibration.

3. Single particle cryo-electron microscopy and 3-D reconstruction of viruses.

4. Fourier-Bessel reconstruction of helical assemblies.

5. Images of paraffin monolayer crystals with perfect contrast: minimization of beam-induced specimen motion.

6. Structure of rhomboid protease in a lipid environment.

7. Three-dimensional structure of TspO by electron cryomicroscopy of helical crystals.

8. Electron crystallography reveals plasticity within the drug binding site of the small multidrug transporter EmrE.

9. Detective quantum efficiency of electron area detectors in electron microscopy.

Review 10. Structural analysis of macromolecular assemblies by electron microscopy.