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Literature DB >> 16837195

Development of a fluorescent intercalator displacement assay (G4-FID) for establishing quadruplex-DNA affinity and selectivity of putative ligands.

David Monchaud¹, Clémence Allain, Marie-Paule Teulade-Fichou.

Abstract

A fluorescent intercalator displacement assay (G4-FID) has been designed based on the displacement of thiazole orange (TO) positioned onto a quadruplex-forming oligonucleotide by putative ligands. This technique was validated by the use of a set of representative and fully characterized G-quadruplex binders (ranging from pyridodicarboxamide to macrocyclic ligands). To further extend its applicability, a comparative version has been developed which allows a rapid and viable determination of quadruplex- over duplex-selectivity.

Entities: Chemical

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Year: 2006 PMID： 16837195 DOI： 10.1016/j.bmcl.2006.06.067

Source DB: PubMed Journal: Bioorg Med Chem Lett ISSN： 0960-894X Impact factor: 2.823

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Cited

35 in total

1. Interaction of hemin with quadruplex DNA.

Authors: Maryam Ghahremani Nasab; Leila Hassani; Sara Mohammadi Nejad; Davood Norouzi
Journal: J Biol Phys Date: 2016-10-17 Impact factor: 1.365

2. A rapid fluorescent indicator displacement assay and principal component/cluster data analysis for determination of ligand-nucleic acid structural selectivity.

Authors: Rafael Del Villar-Guerra; Robert D Gray; John O Trent; Jonathan B Chaires
Journal: Nucleic Acids Res Date: 2018-04-20 Impact factor: 16.971

3. Long repeating (TTAGGG) _n single-stranded DNA self-condenses into compact beaded filaments stabilized by G-quadruplex formation.

Authors: Anirban Kar; Nathan Jones; N Özlem Arat; Richard Fishel; Jack D Griffith
Journal: J Biol Chem Date: 2018-04-19 Impact factor: 5.157

4. Synthesis of naphthalimide derivatives with potential anticancer activity, their comparative ds- and G-quadruplex-DNA binding studies and related biological activities.

Authors: Ufuk Yildiz; Irfan Kandemir; Füsun Cömert; Senem Akkoç; Burak Coban
Journal: Mol Biol Rep Date: 2020-02-25 Impact factor: 2.316

Development of a fluorescent intercalator displacement assay (G4-FID) for establishing quadruplex-DNA affinity and selectivity of putative ligands.

1. Interaction of hemin with quadruplex DNA.

2. A rapid fluorescent indicator displacement assay and principal component/cluster data analysis for determination of ligand-nucleic acid structural selectivity.

3. Long repeating (TTAGGG) _n single-stranded DNA self-condenses into compact beaded filaments stabilized by G-quadruplex formation.

4. Synthesis of naphthalimide derivatives with potential anticancer activity, their comparative ds- and G-quadruplex-DNA binding studies and related biological activities.

5. Aminoglycoside binding to Oxytricha nova telomeric DNA.

6. Neomycin-neomycin dimer: an all-carbohydrate scaffold with high affinity for AT-rich DNA duplexes.

7. "One ring to bind them all"-part I: the efficiency of the macrocyclic scaffold for g-quadruplex DNA recognition.

8. Genetic instability triggered by G-quadruplex interacting Phen-DC compounds in Saccharomyces cerevisiae.

Review 9. Modulation of DNA structure formation using small molecules.

10. An assay for human telomeric G-quadruplex DNA binding drugs.