Literature DB >> 16820688

Crystallization and preliminary X-ray diffraction studies of ferredoxin reductase from Leptospira interrogans.

Alessandro S Nascimento1, Thiago Ferrarezi, Daniela L Catalano-Dupuy, Eduardo A Ceccarelli, Igor Polikarpov.   

Abstract

Ferredoxin-NADP+ reductase (FNR) is an FAD-containing enzyme that catalyzes electron transfer between NADP(H) and ferredoxin. Here, results are reported of the recombinant expression, purification and crystallization of FNR from Leptospira interrogans, a parasitic bacterium of animals and humans. The L. interrogans FNR crystals belong to a primitive monoclinic space group and diffract to 2.4 angstroms resolution at a synchrotron source.

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Year:  2006        PMID: 16820688      PMCID: PMC2242957          DOI: 10.1107/S1744309106020136

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN: 1744-3091


  21 in total

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5.  Crystallography & NMR system: A new software suite for macromolecular structure determination.

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6.  Solvent content of protein crystals.

Authors:  B W Matthews
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8.  The ferredoxin-NADP(H) reductase from Rhodobacter capsulatus: molecular structure and catalytic mechanism.

Authors:  Isabel Nogués; Inmaculada Pérez-Dorado; Susana Frago; Cristian Bittel; Stephen G Mayhew; Carlos Gómez-Moreno; Juan A Hermoso; Milagros Medina; Néstor Cortez; Néstor Carrillo
Journal:  Biochemistry       Date:  2005-09-06       Impact factor: 3.162

9.  A productive NADP+ binding mode of ferredoxin-NADP + reductase revealed by protein engineering and crystallographic studies.

Authors:  Z Deng; A Aliverti; G Zanetti; A K Arakaki; J Ottado; E G Orellano; N B Calcaterra; E A Ceccarelli; N Carrillo; P A Karplus
Journal:  Nat Struct Biol       Date:  1999-09

10.  On the choice of an optimal wavelength in macromolecular crystallography.

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  3 in total

1.  A highly stable plastidic-type ferredoxin-NADP(H) reductase in the pathogenic bacterium Leptospira interrogans.

Authors:  Daniela L Catalano-Dupuy; Matías A Musumeci; Arleth López-Rivero; Eduardo A Ceccarelli
Journal:  PLoS One       Date:  2011-10-24       Impact factor: 3.240

2.  Crystal structures of Leptospira interrogans FAD-containing ferredoxin-NADP+ reductase and its complex with NADP+.

Authors:  Alessandro S Nascimento; Daniela L Catalano-Dupuy; Amanda Bernardes; Mario de Oliveira Neto; Maria Auxiliadora M Santos; Eduardo A Ceccarelli; Igor Polikarpov
Journal:  BMC Struct Biol       Date:  2007-10-24

3.  TAT-mediated transduction of bacterial redox proteins generates a cytoprotective effect on neuronal cells.

Authors:  Cecilia L Balaban; Claudia Banchio; Eduardo A Ceccarelli
Journal:  PLoS One       Date:  2017-09-08       Impact factor: 3.240

  3 in total

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