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Literature DB >> 168110

A radioreceptor-assay for glucagon: binding of enteroglucagon to liver plasma membranes.

Abstract

A radioreceptor-assay for glucagon was developed employing pig liver plasma membranes isolated by means of an aqueous two-phase polymer system. The assay is simple, precise, and has a detection limit of 40 pg/ml. Acid-ethanol extracts of porcine intestinal mucosa and entero-glucagon purified by affinity chromatography interfered strongly with the binding of 125I-labelled glucagon. The affinity of enteroglucagon for the membranes was lower than that of glucagon, but even physiological concentrations of the former interfere with glucagon binding, indicating that enteroglucagon may compete with pancreatic glucagon for binding to the hepatocyte under physiological conditions.

Entities: Chemical Disease Species

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Year: 1975 PMID： 168110 DOI： 10.1007/bf00422324

Source DB: PubMed Journal: Diabetologia ISSN： 0012-186X Impact factor: 10.122

36 in total

1. A rapid method for the isolation of rat liver plasma membranes using an aqueous two-phase polymer system.

Authors: L Lesko; M Donlon; G V Marinetti; J D Hare
Journal: Biochim Biophys Acta Date: 1973-06-22

2. Relation of glucagon-specific binding sites to glucagon-dependent stimulation of adenylyl cyclase activity in plasma membranes of rat liver.

Authors: L Birnbaumer; S L Pohl
Journal: J Biol Chem Date: 1973-03-25 Impact factor: 5.157

3. A modified method for the isolation of the plasma membrane from rat liver.

Authors: T K Ray
Journal: Biochim Biophys Acta Date: 1970-01-06

4. Enteroglucagon release in the dumping syndrome.

Authors: S R Bloom; C M Royston; J P Thomson
Journal: Lancet Date: 1972-10-14 Impact factor: 79.321

5. A new method for the isolation of plasma mebranes from pig liver. A combined procedure using 50 Hz-vibration of a sucrose gradient.

Authors: F Lutz; M Frimmer
Journal: Hoppe Seylers Z Physiol Chem Date: 1970-12

6. Glucagon structure and function. II. Increased activity of iodoglucagon.

Authors: W W Bromer; M E Boucher; J M Patterson
Journal: Biochem Biophys Res Commun Date: 1973-07-02 Impact factor: 3.575

7. Specific biologic effects of intestinal glucagon-like materials.

Authors: R A Gutman; G Fink; N Voyles; H Selawry; J C Penhos; A Lepp; L Recant
Journal: J Clin Invest Date: 1973-05 Impact factor: 14.808

8. Insulin immunoassay by back-titration; some characteristics of the technic and the insulin precipitant action of alcohol.

Authors: P H Wright; D R Makulu; D Vichick; K E Sussman
Journal: Diabetes Date: 1971-01 Impact factor: 9.461

9. The reaction of glucagon with its receptor: evidence for discrete regions of activity and binding in the glucagon molecule.

Authors: M Rodbell; L Birnbaumer; S L Pohl; F Sundby
Journal: Proc Natl Acad Sci U S A Date: 1971-05 Impact factor: 11.205

A radioreceptor-assay for glucagon: binding of enteroglucagon to liver plasma membranes.

1. A rapid method for the isolation of rat liver plasma membranes using an aqueous two-phase polymer system.

2. Relation of glucagon-specific binding sites to glucagon-dependent stimulation of adenylyl cyclase activity in plasma membranes of rat liver.

3. A modified method for the isolation of the plasma membrane from rat liver.

4. Enteroglucagon release in the dumping syndrome.

5. A new method for the isolation of plasma mebranes from pig liver. A combined procedure using 50 Hz-vibration of a sucrose gradient.

6. Glucagon structure and function. II. Increased activity of iodoglucagon.

7. Specific biologic effects of intestinal glucagon-like materials.

8. Insulin immunoassay by back-titration; some characteristics of the technic and the insulin precipitant action of alcohol.

9. The reaction of glucagon with its receptor: evidence for discrete regions of activity and binding in the glucagon molecule.

10. Radioreceptor assay of adrenocorticotropic hormone: new approach to assay of polypeptide hormones in plasma.

Review 1. Interactions polypeptide hormones with cell membrane specific receptors: studies with insulin and glucagon.

2. The effect of induced hyperglucagonaemia on the Zucker fatty rat.

3. Evidence that enteroglucagon (II) is identical with the C-terminal sequence (residues 33-69) of glicentin.

4. Molecular heterogeneity of glucagon in normal subjects and in patients with glucagon-producing tumours.

5. Extraction, gel filtration pattern, and receptor binding of porcine gastrointestinal glucagon-like immunoreactivity.