Literature DB >> 16802025

Immobilization of proteins on agarose beads, monitored in real time by bead injection spectroscopy.

Jaromir Ruzicka1, Andrea D Carroll, Ilkka Lähdesmäki.   

Abstract

A novel approach to real-time monitoring of protein immobilization resulted in the surprising finding that current immobilization protocols are far from optimized.

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Year:  2006        PMID: 16802025      PMCID: PMC1585966          DOI: 10.1039/b603768b

Source DB:  PubMed          Journal:  Analyst        ISSN: 0003-2654            Impact factor:   4.616


  6 in total

Review 1.  Novel flow injection methods for drug-receptor interaction studies, based on probing cell metabolism.

Authors:  I Lähdesmäki; J Ruzicka; A Ivaska
Journal:  Analyst       Date:  2000-10       Impact factor: 4.616

2.  Label dilution method: a novel tool for bioligand interaction studies using bead injection in the lab-on-valve format.

Authors:  Andrea D Carroll; Louis Scampavia; Jaromir Ruzicka
Journal:  Analyst       Date:  2002-09       Impact factor: 4.616

3.  Real-time monitoring of lactate extrusion and glucose consumption of cultured cells using a lab-on-valve system.

Authors:  Craig M Schulz; Louis Scampavia; Jaromir Ruzicka
Journal:  Analyst       Date:  2002-12       Impact factor: 4.616

4.  Site-directed immobilization of proteins.

Authors:  P L Domen; J R Nevens; A K Mallia; G T Hermanson; D C Klenk
Journal:  J Chromatogr       Date:  1990-06-27

5.  Bioligand interaction assay by flow injection absorptiometry.

Authors:  J Ruzicka; A Ivaska
Journal:  Anal Chem       Date:  1997-12-15       Impact factor: 6.986

6.  Selective enzyme purification by affinity chromatography.

Authors:  P Cuatrecasas; M Wilchek; C B Anfinsen
Journal:  Proc Natl Acad Sci U S A       Date:  1968-10       Impact factor: 11.205

  6 in total
  3 in total

1.  Two-parameter monitoring in a lab-on-valve manifold, applied to intracellular H2O2 measurements.

Authors:  Ilkka Lähdesmäki; Petr Chocholous; Andrea D Carroll; Judy Anderson; Peter S Rabinovitch; Jaromir Ruzicka
Journal:  Analyst       Date:  2009-04-27       Impact factor: 4.616

Review 2.  Chemoenzymatic method for glycomics: Isolation, identification, and quantitation.

Authors:  Shuang Yang; Abigail Rubin; Shadi Toghi Eshghi; Hui Zhang
Journal:  Proteomics       Date:  2015-11-06       Impact factor: 3.984

3.  Co-Immobilization of RizA Variants with Acetate Kinase for the Production of Bioactive Arginyl Dipeptides.

Authors:  Sven Bordewick; Ralf G Berger; Franziska Ersoy
Journal:  Molecules       Date:  2022-07-07       Impact factor: 4.927

  3 in total

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