| Literature DB >> 16799556 |
Roman K Thomas1, Elizabeth Nickerson, Jan F Simons, Pasi A Jänne, Torstein Tengs, Yuki Yuza, Levi A Garraway, Thomas LaFramboise, Jeffrey C Lee, Kinjal Shah, Keith O'Neill, Hidefumi Sasaki, Neal Lindeman, Kwok-Kin Wong, Ana M Borras, Edward J Gutmann, Konstantin H Dragnev, Ralph DeBiasi, Tzu-Hsiu Chen, Karen A Glatt, Heidi Greulich, Brian Desany, Christine K Lubeski, William Brockman, Pablo Alvarez, Stephen K Hutchison, J H Leamon, Michael T Ronan, Gregory S Turenchalk, Michael Egholm, William R Sellers, Jonathan M Rothberg, Matthew Meyerson.
Abstract
The sensitivity of conventional DNA sequencing in tumor biopsies is limited by stromal contamination and by genetic heterogeneity within the cancer. Here, we show that microreactor-based pyrosequencing can detect rare cancer-associated sequence variations by independent and parallel sampling of multiple representatives of a given DNA fragment. This technology can thereby facilitate accurate molecular diagnosis of heterogeneous cancer specimens and enable patient selection for targeted cancer therapies.Entities:
Mesh：
Substances：
Year: 2006 PMID： 16799556 DOI： 10.1038/nm1437
Source DB: PubMed Journal: Nat Med ISSN： 1078-8956 Impact factor: 53.440