Literature DB >> 16793282

Application of photobleaching for measuring diffusion of prion proteins in cytosol of yeast cells.

Yue-Xuan Wu1, Daniel C Masison, Evan Eisenberg, Lois E Greene.   

Abstract

Measurement of fluorescence recovery after photobleaching (FRAP) is a non-invasive technique for studying protein dynamics in real time in living cells. FRAP studies are carried out on proteins tagged with green fluorescent protein (GFP) or one of its spectral variants. Illumination with high intensity laser light irreversibly bleaches the GFP fluorescence but has no effect on protein function. By photobleaching a limited region of the cytoplasm, the rate of fluorescence recovery provides a measure of the rate of protein diffusion. A detailed description of the FRAP technique is given, including its application to measuring the mobility of GFP-tagged Sup35p in [psi(-)] and [PSI(+)] cells.

Entities:  

Mesh：

Substances：

Year:  2006        PMID： 16793282      PMCID： PMC3169331          DOI： 10.1016/j.ymeth.2006.04.004

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  14 in total

1.  Measurement of molecular diffusion in solution by multiphoton fluorescence photobleaching recovery.

Authors:  E B Brown; E S Wu; W Zipfel; W W Webb
Journal:  Biophys J       Date:  1999-11       Impact factor: 4.033

2.  Prions affect the appearance of other prions: the story of [PIN(+)].

Authors:  I L Derkatch; M E Bradley; J Y Hong; S W Liebman
Journal:  Cell       Date:  2001-07-27       Impact factor: 41.582

Review 3.  Studying protein dynamics in living cells.

Authors:  J Lippincott-Schwartz; E Snapp; A Kenworthy
Journal:  Nat Rev Mol Cell Biol       Date:  2001-06       Impact factor: 94.444

Review 4.  From fixed to FRAP: measuring protein mobility and activity in living cells.

Authors:  E A Reits; J J Neefjes
Journal:  Nat Cell Biol       Date:  2001-06       Impact factor: 28.824

Review 5.  Photobleaching and photoactivation: following protein dynamics in living cells.

Authors:  Jennifer Lippincott-Schwartz; Nihal Altan-Bonnet; George H Patterson
Journal:  Nat Cell Biol       Date:  2003-09       Impact factor: 28.824

6.  Molecular analysis of microscopic ezrin dynamics by two-photon FRAP.

Authors:  Sylvie Coscoy; François Waharte; Alexis Gautreau; Marianne Martin; Daniel Louvard; Paul Mangeat; Monique Arpin; Françis Amblard
Journal:  Proc Natl Acad Sci U S A       Date:  2002-09-23       Impact factor: 11.205

7.  Hsp70 dynamics in vivo: effect of heat shock and protein aggregation.

Authors:  Xian-Chun Zeng; Samir Bhasin; Xufeng Wu; Joeng-Goo Lee; Shivani Maffi; Christopher J Nichols; Kyung Jin Lee; J Paul Taylor; Lois E Greene; Evan Eisenberg
Journal:  J Cell Sci       Date:  2004-09-14       Impact factor: 5.285

8.  Green fluorescent protein as a marker for gene expression.

Authors:  M Chalfie; Y Tu; G Euskirchen; W W Ward; D C Prasher
Journal:  Science       Date:  1994-02-11       Impact factor: 47.728

9.  Aggregation of huntingtin in yeast varies with the length of the polyglutamine expansion and the expression of chaperone proteins.

Authors:  S Krobitsch; S Lindquist
Journal:  Proc Natl Acad Sci U S A       Date:  2000-02-15       Impact factor: 11.205

10.  Polyglutamine protein aggregates are dynamic.

Authors:  Soojin Kim; Ellen A A Nollen; Kazunori Kitagawa; Vytautas P Bindokas; Richard I Morimoto
Journal:  Nat Cell Biol       Date:  2002-10       Impact factor: 28.213
View more
  10 in total

1.  Localization of HET-S to the cell periphery, not to [Het-s] aggregates, is associated with [Het-s]-HET-S toxicity.

Authors:  Vidhu Mathur; Carolin Seuring; Roland Riek; Sven J Saupe; Susan W Liebman
Journal:  Mol Cell Biol       Date:  2011-10-28       Impact factor: 4.272

2.  Dynamic SpoIIIE assembly mediates septal membrane fission during Bacillus subtilis sporulation.

Authors:  Tinya C Fleming; Jae Yen Shin; Sang-Hyuk Lee; Eric Becker; Kerwyn Casey Huang; Carlos Bustamante; Kit Pogliano
Journal:  Genes Dev       Date:  2010-06-01       Impact factor: 11.361

3.  Light-guided surface engineering for biomedical applications.

Authors:  Ashwath Jayagopal; Gregory P Stone; Frederick R Haselton
Journal:  Bioconjug Chem       Date:  2008-03-04       Impact factor: 4.774

4.  Dissecting protein reaction dynamics in living cells by fluorescence recovery after photobleaching.

Authors:  Marco Fritzsche; Guillaume Charras
Journal:  Nat Protoc       Date:  2015-04-02       Impact factor: 13.491

5.  Visualization of pinholin lesions in vivo.

Authors:  Ting Pang; Tinya C Fleming; Kit Pogliano; Ry Young
Journal:  Proc Natl Acad Sci U S A       Date:  2013-05-13       Impact factor: 11.205

6.  Heat shock protein 104 (Hsp104)-mediated curing of [PSI+] yeast prions depends on both [PSI+] conformation and the properties of the Hsp104 homologs.

Authors:  Xiaohong Zhao; Ramon Rodriguez; Rebecca E Silberman; Joseph M Ahearn; Sheela Saidha; Kaelyn C Cummins; Evan Eisenberg; Lois E Greene
Journal:  J Biol Chem       Date:  2017-04-03       Impact factor: 5.157

Review 7.  Application of GFP-labeling to study prions in yeast.

Authors:  Lois E Greene; Yang-Nim Park; Daniel C Masison; Evan Eisenberg
Journal:  Protein Pept Lett       Date:  2009       Impact factor: 1.890

8.  Tomato UDP-Glucose Sterol Glycosyltransferases: A Family of Developmental and Stress Regulated Genes that Encode Cytosolic and Membrane-Associated Forms of the Enzyme.

Authors:  Karla Ramirez-Estrada; Nídia Castillo; Juan A Lara; Monserrat Arró; Albert Boronat; Albert Ferrer; Teresa Altabella
Journal:  Front Plant Sci       Date:  2017-06-09       Impact factor: 5.753

9.  Hsp70 targets Hsp100 chaperones to substrates for protein disaggregation and prion fragmentation.

Authors:  Juliane Winkler; Jens Tyedmers; Bernd Bukau; Axel Mogk
Journal:  J Cell Biol       Date:  2012-08-06       Impact factor: 10.539

10.  Identification and Characterization of Sterol Acyltransferases Responsible for Steryl Ester Biosynthesis in Tomato.

Authors:  Juan A Lara; Alma Burciaga-Monge; Angel Chávez; Marc Revés; Rodolfo Lavilla; Monserrat Arró; Albert Boronat; Teresa Altabella; Albert Ferrer
Journal:  Front Plant Sci       Date:  2018-05-08       Impact factor: 5.753
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.