Literature DB >> 12271120

Molecular analysis of microscopic ezrin dynamics by two-photon FRAP.

Sylvie Coscoy1, François Waharte, Alexis Gautreau, Marianne Martin, Daniel Louvard, Paul Mangeat, Monique Arpin, Françis Amblard.   

Abstract

Ezrin plays a key role in coupling signal transduction to cortical cell organization. This actin-membrane linker undergoes a series of conformational changes that modulate its interactions with various partners and its localization in membrane or cytosolic pools. Its mobility and exchange rates within and between these two pools were assessed by two-photon fluorescence recovery after photobleaching in epithelial cell microvilli. Analysis of ezrin mutants with an altered actin-binding site revealed three ezrin membrane states of different mobilities and exchange properties, reflecting sequential association with membrane components and F-actin in the context of a fast overall turnover.

Entities:  

Mesh：

Substances：

Year:  2002        PMID： 12271120      PMCID： PMC130542          DOI： 10.1073/pnas.192084599

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  26 in total

1.  Measurement of molecular diffusion in solution by multiphoton fluorescence photobleaching recovery.

Authors:  E B Brown; E S Wu; W Zipfel; W W Webb
Journal:  Biophys J       Date:  1999-11       Impact factor: 4.033

2.  In vivo functional analysis of ezrin during mouse blastocyst formation.

Authors:  N Dard; S Louvet; A Santa-Maria; J Aghion; M Martin; P Mangeat; B Maro
Journal:  Dev Biol       Date:  2001-05-01       Impact factor: 3.582

3.  Reconstituted syntaxin1a/SNAP25 interacts with negatively charged lipids as measured by lateral diffusion in planar supported bilayers.

Authors:  M L Wagner; L K Tamm
Journal:  Biophys J       Date:  2001-07       Impact factor: 4.033

Review 4.  ERM proteins in cell adhesion and membrane dynamics.

Authors:  P Mangeat; C Roy; M Martin
Journal:  Trends Cell Biol       Date:  1999-05       Impact factor: 20.808

5.  Tyrosine phosphorylation and cellular redistribution of ezrin in MDCK cells treated with pervanadate.

Authors:  Y X Wu; T Uezato; M Fujita
Journal:  J Cell Biochem       Date:  2000-08-02       Impact factor: 4.429

Review 6.  ERM-Merlin and EBP50 protein families in plasma membrane organization and function.

Authors:  A Bretscher; D Chambers; R Nguyen; D Reczek
Journal:  Annu Rev Cell Dev Biol       Date:  2000       Impact factor: 13.827

7.  Direct involvement of ezrin/radixin/moesin (ERM)-binding membrane proteins in the organization of microvilli in collaboration with activated ERM proteins.

Authors:  S Yonemura; S Tsukita; S Tsukita
Journal:  J Cell Biol       Date:  1999-06-28       Impact factor: 10.539

8.  Morphogenic effects of ezrin require a phosphorylation-induced transition from oligomers to monomers at the plasma membrane.

Authors:  A Gautreau; D Louvard; M Arpin
Journal:  J Cell Biol       Date:  2000-07-10       Impact factor: 10.539

9.  Mutagenesis of the phosphatidylinositol 4,5-bisphosphate (PIP(2)) binding site in the NH(2)-terminal domain of ezrin correlates with its altered cellular distribution.

Authors:  C Barret; C Roy; P Montcourrier; P Mangeat; V Niggli
Journal:  J Cell Biol       Date:  2000-11-27       Impact factor: 10.539

10.  Active protein transport through plastid tubules: velocity quantified by fluorescence correlation spectroscopy.

Authors:  R H Köhler; P Schwille; W W Webb; M R Hanson
Journal:  J Cell Sci       Date:  2000-11       Impact factor: 5.285
View more
  51 in total

1.  Analysis of binding reactions by fluorescence recovery after photobleaching.

Authors:  Brian L Sprague; Robert L Pego; Diana A Stavreva; James G McNally
Journal:  Biophys J       Date:  2004-06       Impact factor: 4.033

2.  FRAP analysis of membrane-associated proteins: lateral diffusion and membrane-cytoplasmic exchange.

Authors:  Nathan W Goehring; Debanjan Chowdhury; Anthony A Hyman; Stephan W Grill
Journal:  Biophys J       Date:  2010-10-20       Impact factor: 4.033

3.  Dissecting the contribution of diffusion and interactions to the mobility of nuclear proteins.

Authors:  Joël Beaudouin; Felipe Mora-Bermúdez; Thorsten Klee; Nathalie Daigle; Jan Ellenberg
Journal:  Biophys J       Date:  2005-12-30       Impact factor: 4.033

4.  Application of photobleaching for measuring diffusion of prion proteins in cytosol of yeast cells.

Authors:  Yue-Xuan Wu; Daniel C Masison; Evan Eisenberg; Lois E Greene
Journal:  Methods       Date:  2006-05       Impact factor: 3.608

5.  Differences in protein mobility between pioneer versus follower growth cones.

Authors:  Rajan P Kulkarni; Magdalena Bak-Maier; Scott E Fraser
Journal:  Proc Natl Acad Sci U S A       Date:  2007-01-17       Impact factor: 11.205

6.  Structural plasticity of perisynaptic astrocyte processes involves ezrin and metabotropic glutamate receptors.

Authors:  Monique Lavialle; Georg Aumann; Enrico Anlauf; Felicitas Pröls; Monique Arpin; Amin Derouiche
Journal:  Proc Natl Acad Sci U S A       Date:  2011-07-13       Impact factor: 11.205

7.  Atypical protein kinase C (iota) activates ezrin in the apical domain of intestinal epithelial cells.

Authors:  Flavia A Wald; Andrea S Oriolo; Anastasia Mashukova; Nevis L Fregien; Amber H Langshaw; Pedro J I Salas
Journal:  J Cell Sci       Date:  2008-02-12       Impact factor: 5.285

8.  Endocytosis is required for E-cadherin redistribution at mature adherens junctions.

Authors:  Simon de Beco; Charles Gueudry; François Amblard; Sylvie Coscoy
Journal:  Proc Natl Acad Sci U S A       Date:  2009-04-16       Impact factor: 11.205

9.  Metabolic imaging in multiple time scales.

Authors:  V Krishnan Ramanujan
Journal:  Methods       Date:  2013-09-04       Impact factor: 3.608

10.  Kinetic and mechanical analysis of live tube morphogenesis.

Authors:  Alan M Cheshire; Bilal E Kerman; Warren R Zipfel; Alexander A Spector; Deborah J Andrew
Journal:  Dev Dyn       Date:  2008-10       Impact factor: 3.780
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.