| Literature DB >> 16793202 |
Wichuda Bamrungphon1, Nalinee Prempracha, Nophawan Bunchu, Samreung Rangdaeng, Trichak Sandhu, Songphol Srisukho, Chanchai Boonla, Sopit Wongkham.
Abstract
We have previously used agarose gel electrophoresis and immunoblotting to qualitatively measure serum MUC5AC mucin for diagnosing cholangiocarcinoma. In this study, we developed a quantitative determination of serum MUC5AC by sandwich ELISA using MUC5AC mucin monoclonal antibody and soybean agglutinin. A cut-off value of the absorbance 0.074 was obtained from a complete statistical Receiver Operating Characteristic curves with an area under the curve=0.8141. The assay could discriminate cholangiocarcinoma patients from the controls with 71% sensitivity and 90% specificity. The test is simple to perform, reproducible, and probably used for detecting cholangiocarcinoma in a high-risk group or suspected patients.Entities:
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Year: 2006 PMID： 16793202 DOI： 10.1016/j.canlet.2006.05.007
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679