Literature DB >> 16791494

The Calibration Kit Spectral Fluorescence Standards--a simple and certified tool for the standardization of the spectral characteristics of fluorescence instruments.

D Pfeifer1, K Hoffmann, A Hoffmann, C Monte, U Resch-Genger.   

Abstract

With the Calibration Kit Spectral Fluorescence Standards BAM-F001-BAM-F005, we developed a simple tool for the characterization of the relative spectral responsivity and the long-term stability of the emission channel of fluorescence instruments under routine measurement conditions thereby providing the basis for an improved comparability of fluorescence measurements and eventually standardization. This first set of traceable fluorescence standards, which links fluorescence measurements to the spectral radiance scale in the spectral range of 300-770 nm and has been optimized for spectrofluorometers, can be employed for different measurement geometries and can be adapted to different fluorescence techniques with proper consideration of the underlying measurement principles.

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Year:  2006        PMID: 16791494     DOI: 10.1007/s10895-006-0086-8

Source DB:  PubMed          Journal:  J Fluoresc        ISSN: 1053-0509            Impact factor:   2.217


  5 in total

1.  Corrected emission spectra and quantum yields for a series of fluorescent compounds in the visible spectral region.

Authors:  Rance A Velapoldi; Hanne H Tønnesen
Journal:  J Fluoresc       Date:  2004-07       Impact factor: 2.217

2.  How to improve quality assurance in fluorometry: fluorescence-inherent sources of error and suited fluorescence standards.

Authors:  U Resch-Genger; K Hoffmann; W Nietfeld; A Engel; J Neukammer; R Nitschke; B Ebert; R Macdonald
Journal:  J Fluoresc       Date:  2005-05       Impact factor: 2.217

3.  Traceability in fluorometry--Part I: Physical standards.

Authors:  J Hollandt; R D Taubert; J Seidel; U Resch-Genger; A Gugg-Helminger; D Pfeifer; C Monte; W Pilz
Journal:  J Fluoresc       Date:  2005-05       Impact factor: 2.217

4.  Traceability in fluorometry: Part II. Spectral fluorescence standards.

Authors:  U Resch-Genger; D Pfeifer; C Monte; W Pilz; A Hoffmann; M Spieles; K Rurack; J Hollandt; D Taubert; B Schönenberger; P Nording
Journal:  J Fluoresc       Date:  2005-05       Impact factor: 2.217

5.  Standardization of fluorescence spectra and the calibration of spectrofluorimeters.

Authors:  J W Eastman
Journal:  Appl Opt       Date:  1966-07-01       Impact factor: 1.980

  5 in total
  6 in total

1.  Relative and absolute determination of fluorescence quantum yields of transparent samples.

Authors:  Christian Würth; Markus Grabolle; Jutta Pauli; Monika Spieles; Ute Resch-Genger
Journal:  Nat Protoc       Date:  2013-07-18       Impact factor: 13.491

2.  Novel fluorophores as building blocks for optical probes for in vivo near infrared fluorescence (NIRF) imaging.

Authors:  Jutta Pauli; Robert Brehm; Monika Spieles; Werner A Kaiser; Ingrid Hilger; Ute Resch-Genger
Journal:  J Fluoresc       Date:  2010-03-06       Impact factor: 2.217

3.  Polymer-and glass-based fluorescence standards for the near infrared (NIR) spectral region.

Authors:  Christian Würth; Katrin Hoffmann; Thomas Behnke; Marius Ohnesorge; Ute Resch-Genger
Journal:  J Fluoresc       Date:  2011-05       Impact factor: 2.217

4.  Mg2+-dependent conformational changes and product release during DNA-catalyzed RNA ligation monitored by Bimane fluorescence.

Authors:  Elisa Turriani; Claudia Höbartner; Thomas M Jovin
Journal:  Nucleic Acids Res       Date:  2014-12-10       Impact factor: 16.971

5.  Sensitive detection of p65 homodimers using red-shifted and fluorescent protein-based FRET couples.

Authors:  Joachim Goedhart; Joop E M Vermeer; Merel J W Adjobo-Hermans; Laura van Weeren; Theodorus W J Gadella
Journal:  PLoS One       Date:  2007-10-10       Impact factor: 3.240

6.  Global effects of agriculture on fluvial dissolved organic matter.

Authors:  Daniel Graeber; Iola G Boëchat; Francisco Encina-Montoya; Carlos Esse; Jörg Gelbrecht; Guillermo Goyenola; Björn Gücker; Marlen Heinz; Brian Kronvang; Mariana Meerhoff; Jorge Nimptsch; Martin T Pusch; Ricky C S Silva; Daniel von Schiller; Elke Zwirnmann
Journal:  Sci Rep       Date:  2015-11-06       Impact factor: 4.379

  6 in total

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