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Literature DB >> 16779646

Stability of soybean recombinant plastome over six generations.

Nathalie Dufourmantel¹, Ghislaine Tissot, Frédéric Garçon, Bernard Pelissier, Manuel Dubald.

Abstract

The stability of a plastid transgene has been evaluated in soybean transformants over six generations. These transformants had integrated the aadA selection cassette in the intergenic region between the rps12/7 and trnV genes. Three independent homoplasmic T0 transformation events were selected and ten plants from each event propagated to generation T5 in the absence of selection pressure. No transgene rearrangement nor wild-type plastome were detected in generation T5 by Southern blot analysis. All tested progenies were uniformly resistant to spectinomycin. Therefore, soybean transformants of generations T0 and T5 appear to be genetically and phenotypically identical.

Entities: Chemical Gene Species

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Year: 2006 PMID： 16779646 DOI： 10.1007/s11248-005-5262-0

Source DB: PubMed Journal: Transgenic Res ISSN： 0962-8819 Impact factor: 2.788

31 in total

1. Efficient elimination of selectable marker genes from the plastid genome by the CRE-lox site-specific recombination system.

Authors: S Corneille; K Lutz; Z Svab; P Maliga
Journal: Plant J Date: 2001-07 Impact factor: 6.417

Stability of soybean recombinant plastome over six generations.

1. Efficient elimination of selectable marker genes from the plastid genome by the CRE-lox site-specific recombination system.

2. Chloroplast transformation in oilseed rape.

Review 3. Plastid transformation in higher plants.

4. Generation and analysis of soybean plastid transformants expressing Bacillus thuringiensis Cry1Ab protoxin.

5. Expression of bar in the plastid genome confers herbicide resistance.

6. Generation of fertile transplastomic soybean.

7. Removal of antibiotic resistance genes from transgenic tobacco plastids.

8. Direct measurement of the transfer rate of chloroplast DNA into the nucleus.

9. Phytoremediation of organomercurial compounds via chloroplast genetic engineering.

10. Transfer of plastid DNA from tobacco to the soil bacterium Acinetobacter sp. by natural transformation.

1. High-level expression of active human alpha1-antitrypsin in transgenic tobacco chloroplasts.