| Literature DB >> 16769110 |
Jung Soo Suk1, Junghae Suh, Kokleong Choy, Samuel K Lai, Jie Fu, Justin Hanes.
Abstract
A number of neurodegenerative disorders may potentially be treated by the delivery of therapeutic genes to neurons. Nonviral gene delivery systems, however, typically provide low transfection efficiency in post-mitotic differentiated neurons. To uncover mechanistic reasons for this observation, we compared gene transfer to undifferentiated and differentiated SH-SY5Y cells using polyethylenimine (PEI)/DNA nanocomplexes. Differentiated cells exhibited substantially lower uptake of gene vectors. To overcome this bottleneck, RGD or HIV-1 Tat peptides were attached to PEI/DNA nanocomplexes via poly(ethylene glycol) (PEG) spacer molecules. Both RGD and Tat improved the cellular uptake of gene vectors and enhanced gene transfection efficiency of primary neurons up to 14-fold. RGD functionalization resulted in a statistically significant increase in vector escape from endosomes, suggesting it may improve gene delivery by more than one mechanism.Entities:
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Year: 2006 PMID： 16769110 PMCID： PMC5737930 DOI： 10.1016/j.biomaterials.2006.05.013
Source DB: PubMed Journal: Biomaterials ISSN： 0142-9612 Impact factor: 12.479