Literature DB >> 16754977

The purification, crystallization and preliminary structural characterization of human MAWDBP, a member of the phenazine biosynthesis-like protein family.

Petra Herde1, Wulf Blankenfeldt.   

Abstract

MAWDBP is the only representative of the phenazine biosynthesis-like protein family in the human genome. Its expression is elevated in several disease processes, including insulin resistance, folate deficiency and hypotension, and it may also be involved in carcinogenesis. The exact molecular function of MAWDBP is unknown. Native and seleno-L-methionine-labelled MAWDBP were expressed in Escherichia coli and crystallized at room temperature from precipitants containing 10 mM KF, 14%(w/v) PEG 3350 and 0.1 M sodium citrate pH 5.4. Crystals belong to space group H32, with unit-cell parameters a = b = 187, c = 241 A, indicative of three to five monomers per asymmetric unit. Crystals were cryoprotected with 15%(v/v) glycerol and data have been collected to 2.7 A resolution.

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Year:  2006        PMID： 16754977      PMCID： PMC2243103          DOI： 10.1107/S1744309106015648

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  22 in total

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Journal:  Cancer Res       Date:  2000-01-01       Impact factor: 12.701

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Authors:  C Iriyama; S Matsuda; R Katsumata; M Hamaguchi
Journal:  J Hum Genet       Date:  2001       Impact factor: 3.172

3.  PCR-array gene expression profiling of hepatocellular carcinoma.

Authors:  Y Kurokawa; R Matoba; S Nakamori; I Takemasa; H Nagano; K Dono; K Umeshita; M Sakon; M Monden; K Kato
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4.  Crystal structure of E. coli yddE protein reveals a striking homology with diaminopimelate epimerase.

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Journal:  Proteins       Date:  2004-05-15

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Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

6.  Amino acid substitutions in structurally related proteins. A pattern recognition approach. Determination of a new and efficient scoring matrix.

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8.  The purification, crystallization and preliminary structural characterization of PhzF, a key enzyme in the phenazine-biosynthesis pathway from Pseudomonas fluorescens 2-79.

Authors:  Dmitri V Mavrodi; Nathalie Bleimling; Linda S Thomashow; Wulf Blankenfeldt
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2003-12-18

9.  Structure and function of the phenazine biosynthesis protein PhzF from Pseudomonas fluorescens 2-79.

Authors:  James F Parsons; Fenhong Song; Lisa Parsons; Kelly Calabrese; Edward Eisenstein; Jane E Ladner
Journal:  Biochemistry       Date:  2004-10-05       Impact factor: 3.162

10.  A graphical user interface to the CCP4 program suite.

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Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2003-06-27
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