| Literature DB >> 16753153 |
Tatsuya Yamamoto1, Shunsuke Izumi, Kunihiko Gekko.
Abstract
The 70S ribosome from Escherichia coli is a supermacro complex (MW: 2.7MDa) comprising three RNA molecules and more than 50 proteins. We have for the first time successfully analyzed the flexibility of 70S ribosomal proteins in solution by detecting the hydrogen/deuterium exchange with mass spectrometry. Based on the deuterium incorporation map of the X-ray structure obtained at the time of each exchange, we demonstrate the structure-flexibility-function relationship of ribosome focusing on the deuterium incorporation of the proteins binding ligands (tRNA, mRNA, and elongation factor) and the relation with structural assembly processes.Entities:
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Year: 2006 PMID： 16753153 DOI： 10.1016/j.febslet.2006.05.049
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124