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Literature DB >> 16737218

Following aptamer-thrombin binding by force measurements.

Bernhard Basnar¹, Roey Elnathan, Itamar Willner.

Abstract

The rupture forces between an aptamer (1)-functionalized AFM tip and a thrombin-modified Au surface are analyzed. The rupture force for a single aptamer/thrombin complex is determined as approximately 4.45 pN. The analysis of the system reveals that the rupture forces correspond to the melting of the G-quadruplex structure of the aptamer bound to the thrombin. This melting of the G-quadruplex leads to the dissociation of the aptamer/thrombin complex.

Entities: Chemical Disease Gene

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Year: 2006 PMID： 16737218 DOI： 10.1021/ac052289e

Source DB: PubMed Journal: Anal Chem ISSN： 0003-2700 Impact factor: 6.986

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Cited

10 in total

1. Ultrasensitive aptamer-based protein detection via a dual amplified biocatalytic strategy.

Authors: Yun Xiang; Yuyong Zhang; Xiaoqing Qian; Yaqin Chai; Joseph Wang; Ruo Yuan
Journal: Biosens Bioelectron Date: 2010-04-14 Impact factor: 10.618

2. A polycatenated DNA scaffold for the one-step assembly of hierarchical nanostructures.

Authors: Yossi Weizmann; Adam B Braunschweig; Ofer I Wilner; Zoya Cheglakov; Itamar Willner
Journal: Proc Natl Acad Sci U S A Date: 2008-04-07 Impact factor: 11.205

Review 3. [Application of atomic force microscopy-based single molecule force spectroscopy in G-quadruplex studies].

Authors: Wenjing Liu; Tong Sun; Ping Zhang; Lin Li; Junhong Lv; Bin Li
Journal: Nan Fang Yi Ke Da Xue Xue Bao Date: 2018-08-30

4. Characterization of enhanced monovalent and bivalent thrombin DNA aptamer binding using single molecule force spectroscopy.

Authors: Isabel Neundlinger; Alexandra Poturnayova; Ivana Karpisova; Christian Rankl; Peter Hinterdorfer; Maja Snejdarkova; Tibor Hianik; Andreas Ebner
Journal: Biophys J Date: 2011-10-05 Impact factor: 4.033

5. An electrochemical chlorpyrifos aptasensor based on the use of a glassy carbon electrode modified with an electropolymerized aptamer-imprinted polymer and gold nanorods.

Authors: Mahmoud Roushani; Azizollah Nezhadali; Zeynab Jalilian
Journal: Mikrochim Acta Date: 2018-11-15 Impact factor: 5.833

Following aptamer-thrombin binding by force measurements.

1. Ultrasensitive aptamer-based protein detection via a dual amplified biocatalytic strategy.

2. A polycatenated DNA scaffold for the one-step assembly of hierarchical nanostructures.

Review 3. [Application of atomic force microscopy-based single molecule force spectroscopy in G-quadruplex studies].

4. Characterization of enhanced monovalent and bivalent thrombin DNA aptamer binding using single molecule force spectroscopy.

5. An electrochemical chlorpyrifos aptasensor based on the use of a glassy carbon electrode modified with an electropolymerized aptamer-imprinted polymer and gold nanorods.

6. A reusable impedimetric aptasensor for detection of thrombin employing a graphite-epoxy composite electrode.

Review 7. Aptamers and Their Significant Role in Cancer Therapy and Diagnosis.

Review 8. G-Quadruplex-Forming Aptamers-Characteristics, Applications, and Perspectives.

9. An aptasensor for the label-free detection of thrombin based on turn-on fluorescent DNA-templated Cu/Ag nanoclusters.

10. Selection of DNA aptamers using atomic force microscopy.