BACKGROUND: Conventional automated cell counters cannot accurately count residual red blood cells (rRBCs) that are often present in various blood products. A two-color flow cytometric method (FC) was validated for detecting and enumerating rRBCs in platelets (PLTs) and mononuclear cell (MNC) products. STUDY DESIGN AND METHODS: PLT and MNC products for PLTs (CD61-fluorescein isothiocyanate) and rRBC (anti-glycophorin A-phycoerythrin) were double stained, and data were acquired with a flow cytometer. Assay linearity, accuracy, and precision were assessed with a standard-dilution series of rRBCs. This assay was used to determine the rRBCs of apheresis PLTs collected with Trima Accel (Gambro BCT) and MNC products collected with the COBE Spectra (Gambro BCT). RESULTS: The linear range of this assay in PLT and MNC products is 10 to 2000 RBCs per microL (R2=0.994). FC had a mean intraassay coefficient of variation of 11.8 percent at 34 RBCs per microL. A standard clinical hematology analyzer overestimated rRBCs in MNC products by 1.59x10(5)+/-0.7x10(5) RBCs per microL. Apheresis PLTs had a median of 17.4 RBCs per microL, with 99.0 percent containing fewer than 90.0 RBCs per microL. CONCLUSIONS: This method for determining rRBCs in blood products is accurate and repeatable with a lower limit of detection adequate to assess currently available blood products. FC should be considered for determining rRBCs in MNC products.
BACKGROUND: Conventional automated cell counters cannot accurately count residual red blood cells (rRBCs) that are often present in various blood products. A two-color flow cytometric method (FC) was validated for detecting and enumerating rRBCs in platelets (PLTs) and mononuclear cell (MNC) products. STUDY DESIGN AND METHODS: PLT and MNC products for PLTs (CD61-fluorescein isothiocyanate) and rRBC (anti-glycophorin A-phycoerythrin) were double stained, and data were acquired with a flow cytometer. Assay linearity, accuracy, and precision were assessed with a standard-dilution series of rRBCs. This assay was used to determine the rRBCs of apheresis PLTs collected with Trima Accel (Gambro BCT) and MNC products collected with the COBE Spectra (Gambro BCT). RESULTS: The linear range of this assay in PLT and MNC products is 10 to 2000 RBCs per microL (R2=0.994). FC had a mean intraassay coefficient of variation of 11.8 percent at 34 RBCs per microL. A standard clinical hematology analyzer overestimated rRBCs in MNC products by 1.59x10(5)+/-0.7x10(5) RBCs per microL. Apheresis PLTs had a median of 17.4 RBCs per microL, with 99.0 percent containing fewer than 90.0 RBCs per microL. CONCLUSIONS: This method for determining rRBCs in blood products is accurate and repeatable with a lower limit of detection adequate to assess currently available blood products. FC should be considered for determining rRBCs in MNC products.
Authors: Joan Cid; Miguel Lozano; Alyssa Ziman; Kamille A West; Kerry L O'Brien; Michael F Murphy; Silvano Wendel; Alejandro Vázquez; Xavier Ortín; Tor A Hervig; Meghan Delaney; Willy A Flegel; Mark H Yazer Journal: Br J Haematol Date: 2014-10-04 Impact factor: 6.998
Authors: Louis Thibault; Marie Joëlle de Grandmont; Marie-Pierre Cayer; Nathalie Dussault; Annie Jacques; Eric Ducas; Annie Beauséjour; André Lebrun Journal: Transfus Med Hemother Date: 2019-06-27 Impact factor: 3.747
Authors: Chloe Cavagnetto; Richard Alejo Blanco; Hollie McKenna; Laura Willmott; Elif Aydogdu; Nicola Akinyemi; Helena Standring; Simon Procter; Johan W Lagerberg; Elin Johansson; Harry Croxon; Dirk de Korte; Stephen F Garner; Atsushi Shirakami; Jarob Saker; Joachim Linssen; Rebecca Cardigan Journal: Transfusion Date: 2020-11-17 Impact factor: 3.157