Literature DB >> 1671746

Expression in Escherichia coli, purification and characterization of two mammalian thioesterases involved in fatty acid synthesis.

J Naggert1, A Witkowski, B Wessa, S Smith.   

Abstract

Thioesterase I, a constituent domain of the multifunctional fatty acid synthase, and thioesterase II, an independent monofunctional protein, catalyse the chain-terminating reaction in fatty acid synthesis de novo at long and medium chain lengths respectively. The enzymes have been cloned and expressed in Escherichia coli under the control of the temperature-sensitive lambda repressor. The recombinant proteins are full-length catalytically competent thioesterases with specificities indistinguishable from those of the natural enzymes.

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Year:  1991        PMID： 1671746      PMCID： PMC1149831          DOI： 10.1042/bj2730787

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  18 in total

1.  Molecular cloning and sequence analysis of complementary DNA encoding rat mammary gland medium-chain S-acyl fatty acid synthetase thio ester hydrolase.

Authors:  R Safford; J de Silva; C Lucas; J H Windust; J Shedden; C M James; C M Sidebottom; A R Slabas; M P Tombs; S G Hughes
Journal:  Biochemistry       Date:  1987-03-10       Impact factor: 3.162

2.  The carboxyl-terminal region of thioesterase II participates in the interaction with fatty acid synthase. Use of electrospray ionization mass spectrometry to identify a carboxyl-terminally truncated form of the enzyme.

Authors:  H E Witkowska; B N Green; S Smith
Journal:  J Biol Chem       Date:  1990-04-05       Impact factor: 5.157

3.  Direct cloning and sequence analysis of enzymatically amplified genomic sequences.

Authors:  S J Scharf; G T Horn; H A Erlich
Journal:  Science       Date:  1986-09-05       Impact factor: 47.728

4.  Interaction of rat mammary gland thioesterase II with fatty acid synthetase is dependent on the presence of acyl chains on the synthetase.

Authors:  J Mikkelsen; A Witkowski; S Smith
Journal:  J Biol Chem       Date:  1987-02-05       Impact factor: 5.157

5.  Cloning and sequencing of the medium-chain S-acyl fatty acid synthetase thioester hydrolase cDNA from rat mammary gland.

Authors:  J Naggert; B Williams; D P Cashman; S Smith
Journal:  Biochem J       Date:  1987-04-15       Impact factor: 3.857

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Complete amino acid sequence of the medium-chain S-acyl fatty acid synthetase thio ester hydrolase from rat mammary gland.

Authors:  Z I Randhawa; S Smith
Journal:  Biochemistry       Date:  1987-03-10       Impact factor: 3.162

8.  Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.

Authors:  S M Hsu; L Raine; H Fanger
Journal:  J Histochem Cytochem       Date:  1981-04       Impact factor: 2.479

9.  Molecular cloning and sequencing of a cDNA encoding the thioesterase domain of the rat fatty acid synthetase.

Authors:  J Naggert; A Witkowski; J Mikkelsen; S Smith
Journal:  J Biol Chem       Date:  1988-01-25       Impact factor: 5.157

10.  Involvement of a thioesterase in the production of short-chain fatty acids in the uropygial glands of mallard ducks (Anas platyrhynchos).

Authors:  M De Renobales; L Rogers; P E Kolattukudy
Journal:  Arch Biochem Biophys       Date:  1980-12       Impact factor: 4.013
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  7 in total

1.  Thyroid hormone responsive protein Spot14 enhances catalysis of fatty acid synthase in lactating mammary epithelium.

Authors:  Michael C Rudolph; Elizabeth A Wellberg; Andrew S Lewis; Kristina L Terrell; Andrea L Merz; N Karl Maluf; Natalie J Serkova; Steven M Anderson
Journal:  J Lipid Res       Date:  2014-04-25       Impact factor: 5.922

2.  Alteration of the specificity and regulation of fatty acid synthesis of Escherichia coli by expression of a plant medium-chain acyl-acyl carrier protein thioesterase.

Authors:  T A Voelker; H M Davies
Journal:  J Bacteriol       Date:  1994-12       Impact factor: 3.490

Review 3.  The type I fatty acid and polyketide synthases: a tale of two megasynthases.

Authors:  Stuart Smith; Shiou-Chuan Tsai
Journal:  Nat Prod Rep       Date:  2007-07-02       Impact factor: 13.423

4.  Crystal Structure and Substrate Specificity of Human Thioesterase 2: INSIGHTS INTO THE MOLECULAR BASIS FOR THE MODULATION OF FATTY ACID SYNTHASE.

Authors:  Melissa K Ritchie; Lynnette C Johnson; Jill E Clodfelter; Charles W Pemble; Brian E Fulp; Cristina M Furdui; Steven J Kridel; W Todd Lowther
Journal:  J Biol Chem       Date:  2015-12-09       Impact factor: 5.157

Review 5.  Regulation of fatty acid biosynthesis in Escherichia coli.

Authors:  K Magnuson; S Jackowski; C O Rock; J E Cronan
Journal:  Microbiol Rev       Date:  1993-09

6.  A novel 2-decenoic acid thioester ameliorates corticosterone-induced depression- and anxiety-like behaviors and normalizes reduced hippocampal signal transduction in treated mice.

Authors:  Shoyo Shibata; Munekazu Iinuma; Hitomi Soumiya; Hidefumi Fukumitsu; Yoshiko Furukawa; Shoei Furukawa
Journal:  Pharmacol Res Perspect       Date:  2015-03-25

7.  Type I fatty acid synthase trapped in the octanoyl-bound state.

Authors:  Alexander Rittner; Karthik S Paithankar; Aaron Himmler; Martin Grininger
Journal:  Protein Sci       Date:  2020-02       Impact factor: 6.725
  7 in total

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