Literature DB >> 16704230

Releasable luciferin-transporter conjugates: tools for the real-time analysis of cellular uptake and release.

Lisa R Jones1, Elena A Goun, Rajesh Shinde, Jonathan B Rothbard, Christopher H Contag, Paul A Wender.   

Abstract

The design, synthesis, and evaluation of conjugates of arginine-rich transporters and luciferin are described that release luciferin only after entry into cells that are stably transfected with luciferase. Each molecule of free luciferin that is released after entry generates a photon that can be measured allowing for real-time quantification of uptake and release in cells. The process provides a method to assay uptake and release of free luciferin as a function of variations in the releasable linker and in the transporter.

Entities:  

Mesh：

Substances：

Year:  2006        PMID： 16704230     DOI： 10.1021/ja0586283

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  36 in total

Review 1.  The design of guanidinium-rich transporters and their internalization mechanisms.

Authors:  Paul A Wender; Wesley C Galliher; Elena A Goun; Lisa R Jones; Thomas H Pillow
Journal:  Adv Drug Deliv Rev       Date:  2007-11-09       Impact factor: 15.470

2.  Toward the Ideal Synthesis and Transformative Therapies: The Roles of Step Economy and Function Oriented Synthesis.

Authors:  Paul A Wender
Journal:  Tetrahedron       Date:  2013-06-07       Impact factor: 2.457

Review 3.  Firefly luciferase: an adenylate-forming enzyme for multicatalytic functions.

Authors:  Satoshi Inouye
Journal:  Cell Mol Life Sci       Date:  2009-10-27       Impact factor: 9.261

4.  Real-time analysis of uptake and bioactivatable cleavage of luciferin-transporter conjugates in transgenic reporter mice.

Authors:  Paul A Wender; Elena A Goun; Lisa R Jones; Thomas H Pillow; Jonathan B Rothbard; Rajesh Shinde; Christopher H Contag
Journal:  Proc Natl Acad Sci U S A       Date:  2007-06-11       Impact factor: 11.205

5.  A molecular method for the delivery of small molecules and proteins across the cell wall of algae using molecular transporters.

Authors:  Joel M Hyman; Erika I Geihe; Brian M Trantow; Bahram Parvin; Paul A Wender
Journal:  Proc Natl Acad Sci U S A       Date:  2012-07-30       Impact factor: 11.205

Review 6.  Non-invasive molecular imaging for preclinical cancer therapeutic development.

Authors:  A C O'Farrell; S D Shnyder; G Marston; P L Coletta; J H Gill
Journal:  Br J Pharmacol       Date:  2013-06       Impact factor: 8.739

7.  In vivo bioluminescence imaging of labile iron accumulation in a murine model of Acinetobacter baumannii infection.

Authors:  Allegra T Aron; Marie C Heffern; Zachery R Lonergan; Mark N Vander Wal; Brian R Blank; Benjamin Spangler; Yaofang Zhang; Hyo Min Park; Andreas Stahl; Adam R Renslo; Eric P Skaar; Christopher J Chang
Journal:  Proc Natl Acad Sci U S A       Date:  2017-11-14       Impact factor: 11.205

8.  Guanidinium-rich, glycerol-derived oligocarbonates: a new class of cell-penetrating molecular transporters that complex, deliver, and release siRNA.

Authors:  Paul A Wender; Melanie A Huttner; Daryl Staveness; Jessica R Vargas; Adele F Xu
Journal:  Mol Pharm       Date:  2015-01-27       Impact factor: 4.939

9.  Cell-Penetrating, Guanidinium-Rich Oligophosphoesters: Effective and Versatile Molecular Transporters for Drug and Probe Delivery.

Authors:  Colin J McKinlay; Robert M Waymouth; Paul A Wender
Journal:  J Am Chem Soc       Date:  2016-03-07       Impact factor: 15.419

10.  Dual Targeting of Intracellular Pathogenic Bacteria with a Cleavable Conjugate of Kanamycin and an Antibacterial Cell-Penetrating Peptide.

Authors:  Anna Brezden; Mohamed F Mohamed; Manish Nepal; John S Harwood; Jerrin Kuriakose; Mohamed N Seleem; Jean Chmielewski
Journal:  J Am Chem Soc       Date:  2016-08-17       Impact factor: 15.419
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.