OBJECTIVE: Trichomoniasis, caused by Trichomonas vaginalis, is a prevalent sexually transmitted infection associated with increased risk of HIV infection. An animal model of T. vaginalis infection would enable scientists to further investigate trichomoniasis. STUDY DESIGN: Seven macaques (4 test vs. 3 control) were enrolled in a 2-week pilot study. Eight additional animals participated in a 2-arm (T. vaginalis vs. sham inoculated) crossover study lasting 5 weeks before treatment. In all, 12 Macaca nemestrina monkeys were challenged with a single intravaginal inoculation of 6.6 to 7.1 x 10(5) trichomonads (ATCC 50148). Vaginal culture (InPouch TV), colposcopy, microbiology, pH, and cervical cytokines were assessed at baseline, day 2, and weekly thereafter. RESULTS: Ten of 12 challenged animals tested positive for trichomoniasis for 2 weeks or longer. One animal tested positive on days 2 and 7 but negative thereafter. Only one animal was not infected. Oral metronidazole treatment (35 mg/kg per day for 3 days) resolved infection in all animals. Trichomoniasis infection did not lead to shifts in vaginal microbiology or pH. CONCLUSIONS: A single T. vaginalis inoculation results in persistent infection in the pigtailed macaque.
OBJECTIVE: Trichomoniasis, caused by Trichomonas vaginalis, is a prevalent sexually transmitted infection associated with increased risk of HIV infection. An animal model of T. vaginalis infection would enable scientists to further investigate trichomoniasis. STUDY DESIGN: Seven macaques (4 test vs. 3 control) were enrolled in a 2-week pilot study. Eight additional animals participated in a 2-arm (T. vaginalis vs. sham inoculated) crossover study lasting 5 weeks before treatment. In all, 12 Macaca nemestrina monkeys were challenged with a single intravaginal inoculation of 6.6 to 7.1 x 10(5) trichomonads (ATCC 50148). Vaginal culture (InPouch TV), colposcopy, microbiology, pH, and cervical cytokines were assessed at baseline, day 2, and weekly thereafter. RESULTS: Ten of 12 challenged animals tested positive for trichomoniasis for 2 weeks or longer. One animal tested positive on days 2 and 7 but negative thereafter. Only one animal was not infected. Oral metronidazole treatment (35 mg/kg per day for 3 days) resolved infection in all animals. Trichomoniasis infection did not lead to shifts in vaginal microbiology or pH. CONCLUSIONS: A single T. vaginalis inoculation results in persistent infection in the pigtailed macaque.
Authors: Tara R Henning; Katherine Butler; Debra Hanson; Gail Sturdevant; Shanon Ellis; Elizabeth M Sweeney; James Mitchell; Frank Deyounks; Christi Phillips; Carol Farshy; Yetunde Fakile; John Papp; W Evan Secor; Harlan Caldwell; Dorothy Patton; Janet M McNicholl; Ellen Kersh Journal: J Infect Dis Date: 2014-04-21 Impact factor: 5.226
Authors: Tara Henning; Yetunde Fakile; Christi Phillips; Elizabeth Sweeney; James Mitchell; Dorothy Patton; Gail Sturdevant; Harlan D Caldwell; W Evan Secor; John Papp; R Michael Hendry; Janet McNicholl; Ellen Kersh Journal: J Med Primatol Date: 2011-08 Impact factor: 0.667
Authors: Sarah V Hadzic; Xiaolei Wang; Jason Dufour; Lara Doyle; Preston A Marx; Andrew A Lackner; Daniel B Paulsen; Ronald S Veazey Journal: Am J Reprod Immunol Date: 2014-02-13 Impact factor: 3.886
Authors: Gwendolyn E Wood; Stefanie L Iverson-Cabral; Dorothy L Patton; Peter K Cummings; Yvonne T Cosgrove Sweeney; Patricia A Totten Journal: Infect Immun Date: 2013-06-03 Impact factor: 3.441