BACKGROUND: Sexually transmitted infections (STIs) are associated with an increased risk of HIV infection. To model the interaction between STIs and HIV infection, we evaluated the capacity of the pigtail macaque model to sustain triple infection with Trichomonas vaginalis, Chlamydia trachomatis, and SHIV(SF162P3). METHODS: Seven SHIV(SF162P3) -infected pigtail macaques were inoculated with T. vaginalis only (n = 2), C. trachomatis only (n = 1), both T. vaginalis and C. trachomatis (n = 2), or control media (no STI; n = 2). Infections were confirmed by culture and/or nucleic acid testing. Genital mucosa was visualized by colposcopy. RESULTS: Characteristic gynecologic signs were observed for both STIs, but not in control animals. Manifestations were most prominent at days 7-10 post-infection. STIs persisted between 4 and 6 weeks and were cleared with antibiotics. CONCLUSIONS: These pilot studies demonstrate the first successful STI-SHIV triple infection of pigtail macaques, with clinical presentation of genital STI symptoms similar to those observed in humans. Published 2011. This article is a U.S. Government work and is in the public domain in the USA.
BACKGROUND: Sexually transmitted infections (STIs) are associated with an increased risk of HIV infection. To model the interaction between STIs and HIV infection, we evaluated the capacity of the pigtail macaque model to sustain triple infection with Trichomonas vaginalis, Chlamydia trachomatis, and SHIV(SF162P3). METHODS: Seven SHIV(SF162P3) -infected pigtail macaques were inoculated with T. vaginalis only (n = 2), C. trachomatis only (n = 1), both T. vaginalis and C. trachomatis (n = 2), or control media (no STI; n = 2). Infections were confirmed by culture and/or nucleic acid testing. Genital mucosa was visualized by colposcopy. RESULTS: Characteristic gynecologic signs were observed for both STIs, but not in control animals. Manifestations were most prominent at days 7-10 post-infection. STIs persisted between 4 and 6 weeks and were cleared with antibiotics. CONCLUSIONS: These pilot studies demonstrate the first successful STI-SHIV triple infection of pigtail macaques, with clinical presentation of genital STI symptoms similar to those observed in humans. Published 2011. This article is a U.S. Government work and is in the public domain in the USA.
Authors: P Wølner-Hanssen; J N Krieger; C E Stevens; N B Kiviat; L Koutsky; C Critchlow; T DeRouen; S Hillier; K K Holmes Journal: JAMA Date: 1989-01-27 Impact factor: 56.272
Authors: M Laga; A Manoka; M Kivuvu; B Malele; M Tuliza; N Nzila; J Goeman; F Behets; V Batter; M Alary Journal: AIDS Date: 1993-01 Impact factor: 4.177
Authors: K A Workowski; C E Stevens; R J Suchland; K K Holmes; D A Eschenbach; M B Pettinger; W E Stamm Journal: Clin Infect Dis Date: 1994-10 Impact factor: 9.079
Authors: Philip E Castle; Ana-Cecilia Rodriguez; Frederick P Bowman; Rolando Herrero; Mark Schiffman; M Concepcion Bratti; Lidia Ana Morera; Danny Schust; Peggy Crowley-Nowick; Allan Hildesheim Journal: Clin Diagn Lab Immunol Date: 2004-03
Authors: Carrie Moore; John Sidney; A Michelle English; Amanda Wriston; Donald F Hunt; Jeffrey Shabanowitz; Scott Southwood; Kate Bradley; Bernard A P Lafont; Bianca R Mothé; Alessandro Sette Journal: Immunogenetics Date: 2012-01-26 Impact factor: 2.846
Authors: Christopher W Peterson; Patrick Younan; Patricia S Polacino; Nicholas J Maurice; Hannah W Miller; Martin Prlic; Keith R Jerome; Ann E Woolfrey; Shiu-Lok Hu; Hans-Peter Kiem Journal: J Med Primatol Date: 2013-10 Impact factor: 0.667
Authors: Tara R Henning; Katherine Butler; Debra Hanson; Gail Sturdevant; Shanon Ellis; Elizabeth M Sweeney; James Mitchell; Frank Deyounks; Christi Phillips; Carol Farshy; Yetunde Fakile; John Papp; W Evan Secor; Harlan Caldwell; Dorothy Patton; Janet M McNicholl; Ellen Kersh Journal: J Infect Dis Date: 2014-04-21 Impact factor: 5.226
Authors: Sarah V Hadzic; Xiaolei Wang; Jason Dufour; Lara Doyle; Preston A Marx; Andrew A Lackner; Daniel B Paulsen; Ronald S Veazey Journal: Am J Reprod Immunol Date: 2014-02-13 Impact factor: 3.886
Authors: James M Smith; Rachna Rastogi; Ryan S Teller; Priya Srinivasan; Pedro M M Mesquita; Umadevi Nagaraja; Janet M McNicholl; R Michael Hendry; Chuong T Dinh; Amy Martin; Betsy C Herold; Patrick F Kiser Journal: Proc Natl Acad Sci U S A Date: 2013-09-16 Impact factor: 11.205
Authors: Gwendolyn E Wood; Stefanie L Iverson-Cabral; Dorothy L Patton; Peter K Cummings; Yvonne T Cosgrove Sweeney; Patricia A Totten Journal: Infect Immun Date: 2013-06-03 Impact factor: 3.441
Authors: Robyn M Engel; Monica Morris; Tara Henning; Jana M Ritter; Tara L Jones; Sharon Dietz; Jessica Ayers; Sundaram A Vishwanathan; Leecresia Jenkins; Sherif Zaki; Dirk Wildemeersch; David Garber; Nathaniel Powell; R Michael Hendry; Janet McNicholl; Ellen N Kersh Journal: J Med Primatol Date: 2013-12-27 Impact factor: 0.667
Authors: Aparajita Chatterjee; Daniel M Ratner; Christopher M Ryan; Patricia J Johnson; Barry R O'Keefe; W Evan Secor; Deborah J Anderson; Phillips W Robbins; John Samuelson Journal: PLoS One Date: 2015-08-07 Impact factor: 3.240