PURPOSE: To measure the ability of protons and gamma-rays to effect cell viability and cell survival of human HTB140 melanoma cells. MATERIALS AND METHODS: Exponentially growing HTB140 cells were irradiated close to the Bragg peak maximum of the 62 MeV protons or with 60Co gamma-rays with single doses, ranging from 8 - 24 Gy. Cell viability using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was evaluated at 6 h, 24 h, 48 h or 7 days after irradiation and clonogenic survival was assessed at 7 days after irradiation. Cell cycle phase redistribution and the level of apoptosis were evaluated at 6 h and 48 h after irradiation. RESULTS: The study of cell viability as a function of time (cell survival progression) and cell survival, using a clonal assay, demonstrated the considerably stronger inactivation effect of protons compared to gamma-rays with a relative biological effectiveness (RBE) of approximately 1.64. Cell cycle phase distribution and apoptosis levels with time enabled us to investigate the development and the character of the damage induced by irradiation. Due to the high radio-resistance of HTB140 cells, cell cycle phase redistribution exhibited only a modest cell accumulation in G2/M phase. Protons but not gamma-rays induced apoptosis. CONCLUSIONS: It appears that protons reduce the number of HTB140 cells by apoptosis as well as by severe DNA damage, while gamma-rays eliminate viable cells primarily by the production of irreparable DNA damage. Protons have an increased RBE relative to gamma-rays.
PURPOSE: To measure the ability of protons and gamma-rays to effect cell viability and cell survival of human HTB140 melanoma cells. MATERIALS AND METHODS: Exponentially growing HTB140 cells were irradiated close to the Bragg peak maximum of the 62 MeV protons or with 60Co gamma-rays with single doses, ranging from 8 - 24 Gy. Cell viability using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was evaluated at 6 h, 24 h, 48 h or 7 days after irradiation and clonogenic survival was assessed at 7 days after irradiation. Cell cycle phase redistribution and the level of apoptosis were evaluated at 6 h and 48 h after irradiation. RESULTS: The study of cell viability as a function of time (cell survival progression) and cell survival, using a clonal assay, demonstrated the considerably stronger inactivation effect of protons compared to gamma-rays with a relative biological effectiveness (RBE) of approximately 1.64. Cell cycle phase distribution and apoptosis levels with time enabled us to investigate the development and the character of the damage induced by irradiation. Due to the high radio-resistance of HTB140 cells, cell cycle phase redistribution exhibited only a modest cell accumulation in G2/M phase. Protons but not gamma-rays induced apoptosis. CONCLUSIONS: It appears that protons reduce the number of HTB140 cells by apoptosis as well as by severe DNA damage, while gamma-rays eliminate viable cells primarily by the production of irreparable DNA damage. Protons have an increased RBE relative to gamma-rays.
Authors: Otilija D Keta; Danijela V Todorović; Tanja M Bulat; Pablo Ga Cirrone; Francesco Romano; Giacomo Cuttone; Ivan M Petrović; Aleksandra M Ristić Fira Journal: Exp Biol Med (Maywood) Date: 2016-09-15
Authors: Žakula Jelena; Korićanac Lela; Keta Otilija; Todorović Danijela; A P Cirrone Giuseppe; Romano Francesco; Cuttone Giacomo; Petrović Ivan; Ristić-Fira Aleksandra Journal: Indian J Med Res Date: 2016-05 Impact factor: 2.375
Authors: Elisabeth Mara; Monika Clausen; Suphalak Khachonkham; Simon Deycmar; Clara Pessy; Wolfgang Dörr; Peter Kuess; Dietmar Georg; Sylvia Gruber Journal: Med Phys Date: 2020-05-15 Impact factor: 4.071
Authors: Aleksandra M Ristić-Fira; Lela B Korićanac; Jelena J Zakula; Lucia M Valastro; Gioacchin Iannolo; Giuseppe Privitera; Giacomo Cuttone; Ivan M Petrović Journal: J Exp Clin Cancer Res Date: 2009-04-09
Authors: Katarzyna Jasińska-Konior; Katarzyna Pochylczuk; Elżbieta Czajka; Marta Michalik; Bożena Romanowska-Dixon; Jan Swakoń; Krystyna Urbańska; Martyna Elas Journal: PLoS One Date: 2017-10-10 Impact factor: 3.240