Literature DB >> 16682775

Crystallization and preliminary X-ray analysis of a U2AF65 variant in complex with a polypyrimidine-tract analogue by use of protein engineering.

E Allen Sickmier1, Katherine E Frato, Clara L Kielkopf.   

Abstract

The large subunit of the essential pre-mRNA splicing factor U2 auxiliary factor (U2AF65) binds the polypyrimidine tract near the 3' splice site of pre-mRNA introns and directs the association of the U2 small nuclear ribonucleoprotein particle (U2 snRNP) of the spliceosome with the pre-mRNA. Protein engineering, in which the flexible linker region connecting tandem RNA-recognition motifs (RRMs) within the U2AF65 RNA-binding domain was partially deleted, allowed successful crystallization of the protein-nucleic acid complex. Cocrystals of a U2AF65 variant with a deoxyuridine dodecamer diffract X-rays to 2.9 angstroms resolution and contain one complex per asymmetric unit.

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Year:  2006        PMID： 16682775      PMCID： PMC2219975          DOI： 10.1107/S1744309106012504

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  21 in total

1.  Differential recognition of the polypyrimidine-tract by the general splicing factor U2AF65 and the splicing repressor sex-lethal.

Authors:  R Singh; H Banerjee; M R Green
Journal:  RNA       Date:  2000-06       Impact factor: 4.942

2.  Co-crystallization of the human nuclear cap-binding complex with a m7GpppG cap analogue using protein engineering.

Authors:  Catherine Mazza; Alexandra Segref; Iain W Mattaj; Stephen Cusack
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2002-11-23

3.  Cloning and domain structure of the mammalian splicing factor U2AF.

Authors:  P D Zamore; J G Patton; M R Green
Journal:  Nature       Date:  1992-02-13       Impact factor: 49.962

4.  Identification, purification, and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factor.

Authors:  P D Zamore; M R Green
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

5.  Splice junctions, branch point sites, and exons: sequence statistics, identification, and applications to genome project.

Authors:  P Senapathy; M B Shapiro; N L Harris
Journal:  Methods Enzymol       Date:  1990       Impact factor: 1.600

6.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

7.  A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly.

Authors:  B Ruskin; P D Zamore; M R Green
Journal:  Cell       Date:  1988-01-29       Impact factor: 41.582

8.  Structure of PTB bound to RNA: specific binding and implications for splicing regulation.

Authors:  Florian C Oberstrass; Sigrid D Auweter; Michèle Erat; Yann Hargous; Anke Henning; Philipp Wenter; Luc Reymond; Batoul Amir-Ahmady; Stefan Pitsch; Douglas L Black; Frédéric H-T Allain
Journal:  Science       Date:  2005-09-23       Impact factor: 47.728

9.  The protein Sex-lethal antagonizes the splicing factor U2AF to regulate alternative splicing of transformer pre-mRNA.

Authors:  J Valcárcel; R Singh; P D Zamore; M R Green
Journal:  Nature       Date:  1993-03-11       Impact factor: 49.962

10.  The conserved RNA recognition motif 3 of U2 snRNA auxiliary factor (U2AF 65) is essential in vivo but dispensable for activity in vitro.

Authors:  Hiren Banerjee; Andrew Rahn; Bharat Gawande; Sabine Guth; Juan Valcarcel; Ravinder Singh
Journal:  RNA       Date:  2004-02       Impact factor: 4.942
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  10 in total

1.  A serendipitous discovery that in situ proteolysis is essential for the crystallization of yeast CPSF-100 (Ydh1p).

Authors:  Corey R Mandel; Damara Gebauer; Hailong Zhang; Liang Tong
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-09-30

2.  Structural basis for polypyrimidine tract recognition by the essential pre-mRNA splicing factor U2AF65.

Authors:  E Allen Sickmier; Katherine E Frato; Haihong Shen; Shanthi R Paranawithana; Michael R Green; Clara L Kielkopf
Journal:  Mol Cell       Date:  2006-07-07       Impact factor: 17.970

3.  Structure-guided U2AF65 variant improves recognition and splicing of a defective pre-mRNA.

Authors:  Anant A Agrawal; Krystle J McLaughlin; Jermaine L Jenkins; Clara L Kielkopf
Journal:  Proc Natl Acad Sci U S A       Date:  2014-11-24       Impact factor: 11.205

4.  An Unusual U2AF2 Inhibits Splicing and Attenuates the Virulence of the Human Protozoan Parasite Entamoeba histolytica.

Authors:  Gretter González-Blanco; Guillermina García-Rivera; Patricia Talmás-Rohana; Ester Orozco; José Manuel Galindo-Rosales; Cristina Vélez; Odila Salucedo-Cárdenas; Elisa Azuara-Liceaga; Mario Alberto Rodríguez-Rodríguez; Tomoyoshi Nozaki; Jesús Valdés
Journal:  Front Cell Infect Microbiol       Date:  2022-06-17       Impact factor: 6.073

5.  Roles of polypyrimidine tract binding proteins in major immediate-early gene expression and viral replication of human cytomegalovirus.

Authors:  Ruth S Cruz Cosme; Yasuhiro Yamamura; Qiyi Tang
Journal:  J Virol       Date:  2009-01-14       Impact factor: 5.103

6.  Structure of the central RNA recognition motif of human TIA-1 at 1.95A resolution.

Authors:  Amit O Kumar; Matthew C Swenson; Matthew M Benning; Clara L Kielkopf
Journal:  Biochem Biophys Res Commun       Date:  2008-01-15       Impact factor: 3.575

7.  Solution conformation and thermodynamic characteristics of RNA binding by the splicing factor U2AF65.

Authors:  Jermaine L Jenkins; Haihong Shen; Michael R Green; Clara L Kielkopf
Journal:  J Biol Chem       Date:  2008-10-08       Impact factor: 5.157

8.  A splice site-sensing conformational switch in U2AF2 is modulated by U2AF1 and its recurrent myelodysplasia-associated mutation.

Authors:  Chandani Warnasooriya; Callen F Feeney; Kholiswa M Laird; Dmitri N Ermolenko; Clara L Kielkopf
Journal:  Nucleic Acids Res       Date:  2020-06-04       Impact factor: 16.971

9.  U2AF65 adapts to diverse pre-mRNA splice sites through conformational selection of specific and promiscuous RNA recognition motifs.

Authors:  Jermaine L Jenkins; Anant A Agrawal; Ankit Gupta; Michael R Green; Clara L Kielkopf
Journal:  Nucleic Acids Res       Date:  2013-02-01       Impact factor: 16.971

10.  An extended U2AF(65)-RNA-binding domain recognizes the 3' splice site signal.

Authors:  Anant A Agrawal; Enea Salsi; Rakesh Chatrikhi; Steven Henderson; Jermaine L Jenkins; Michael R Green; Dmitri N Ermolenko; Clara L Kielkopf
Journal:  Nat Commun       Date:  2016-03-08       Impact factor: 14.919
  10 in total

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