Literature DB >> 1667081

Accurate evaluation of the sizes of DNA fragments (from 30 to 4700 kb) in pulse field gel electrophoresis.

N Crété1, J M Delabar, P M Sinet, N Créau-Goldberg.   

Abstract

Construction of long-range genomic maps by pulse field gel electrophoresis requires optimum resolution of large DNA fragments. Using the transverse-alternating field electrophoresis system, we describe a method to accurately evaluate the sizes of fragments generated by rare-cutter digestions within the 30-4700-kb range. A protocol generating large (greater than 1000 kb) molecules by partial digestion is also reported.

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Year:  1991        PMID： 1667081

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  4 in total

1.  Cytogenetic and molecular analysis of a de novo tandem duplication of chromosome 21.

Authors:  J L Blouin; A Aurias; N Créau-Goldberg; F Apiou; C Alcaide-Loridan; A Bruel; M Prieur; J Kraus; J M Delabar; P M Sinet
Journal:  Hum Genet       Date:  1991-12       Impact factor: 4.132

2.  A high-resolution map of 1.6 Mb in the Down syndrome region: a new map between D21S55 and ETS2.

Authors:  P Gosset; N Crété; G Ait Ghezala; D Théophile; C Van Broeckhoven; C Vayssettes; P M Sinet; N Créau
Journal:  Mamm Genome       Date:  1995-02       Impact factor: 2.957

3.  Partial physical map of human chromosome 21 from fibroblast and lymphocyte DNA.

Authors:  N Crété; J M Delabar; Z Rahmani; M L Yaspo; J Kraus; A Marks; P M Sinet; N Créau-Goldberg
Journal:  Hum Genet       Date:  1993-04       Impact factor: 4.132

4.  New chromosome 21 DNA markers isolated by pulsed field gel electrophoresis from an ETS2-containing Down syndrome chromosomal region.

Authors:  M L Yaspo; N Crété; Z Chettouh; J L Blouin; Z Rahmani; D Stehelin; P M Sinet; N Créau-Goldberg; J M Delabar
Journal:  Hum Genet       Date:  1992-12       Impact factor: 4.132
  4 in total

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