Literature DB >> 16668477

Hygromycin resistance gene cassettes for vector construction and selection of transformed rice protoplasts.

Z Zheng1, A Hayashimoto, Z Li, N Murai.   

Abstract

Hygromycin resistance gene cassettes were designed to facilitate vector construction for plant transformation. Unique EcoRI, Pstl, and Sacll sites in the coding sequence of a hygromycin B phosphotransferase gene (hph) from Escherichia coli were eliminated. The mutated hph genes were used to form gene cassettes flanked by EcoRI-Sacll-Kpnl-Hindlll sites. Hygromycin resistance of wild-type and mutated hph genes was indistinguishable in E. coli and rice protoplast growth assay.

Entities:  

Year:  1991        PMID： 16668477      PMCID： PMC1081085          DOI： 10.1104/pp.97.2.832

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  8 in total

1.  Dual interference of hygromycin B with ribosomal translocation and with aminoacyl-tRNA recognition.

Authors:  M J Cabañas; D Vázquez; J Modolell
Journal:  Eur J Biochem       Date:  1978-06-01

2.  Phenotypic suppression and misreading Saccharomyces cerevisiae.

Authors:  A Singh; D Ursic; J Davies
Journal:  Nature       Date:  1979-01-11       Impact factor: 49.962

3.  Rapid and efficient site-specific mutagenesis without phenotypic selection.

Authors:  T A Kunkel; J D Roberts; R A Zakour
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

4.  A polyethylene glycol-mediated protoplast transformation system for production of fertile transgenic rice plants.

Authors:  A Hayashimoto; Z Li; N Murai
Journal:  Plant Physiol       Date:  1990-07       Impact factor: 8.340

5.  Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes.

Authors:  A González; A Jiménez; D Vázquez; J E Davies; D Schindler
Journal:  Biochim Biophys Acta       Date:  1978-12-21

6.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

7.  Structure and expression of the rice glutelin multigene family.

Authors:  T W Okita; Y S Hwang; J Hnilo; W T Kim; A P Aryan; R Larson; H B Krishnan
Journal:  J Biol Chem       Date:  1989-07-25       Impact factor: 5.157

8.  Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae.

Authors:  L Gritz; J Davies
Journal:  Gene       Date:  1983-11       Impact factor: 3.688
  8 in total
  18 in total

1.  High-efficiency silencing of a beta-glucuronidase gene in rice is correlated with repetitive transgene structure but is independent of DNA methylation.

Authors:  M B Wang; P M Waterhouse
Journal:  Plant Mol Biol       Date:  2000-05       Impact factor: 4.076

2.  Genetic transformation of Dichanthium annulatum (Forssk)--an apomictic tropical forage grass.

Authors:  S J Dalton; A J E Bettany; V Bhat; M G Gupta; K Bailey; E Timms; P Morris
Journal:  Plant Cell Rep       Date:  2003-03-28       Impact factor: 4.570

3.  Multiple mode regulation of a cysteine proteinase gene expression in rice.

Authors:  S L Ho; W F Tong; S M Yu
Journal:  Plant Physiol       Date:  2000-01       Impact factor: 8.340

4.  T-DNA activation tagging as a tool to isolate regulators of a metabolic pathway from a genetically non-tractable plant species.

Authors:  L van der Fits; F Hilliou; J Memelink
Journal:  Transgenic Res       Date:  2001-12       Impact factor: 2.788

5.  Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA.

Authors:  P M Waterhouse; M W Graham; M B Wang
Journal:  Proc Natl Acad Sci U S A       Date:  1998-11-10       Impact factor: 11.205

6.  Cre/lox-mediated site-specific integration of Agrobacterium T-DNA in Arabidopsis thaliana by transient expression of cre.

Authors:  A C Vergunst; P J Hooykaas
Journal:  Plant Mol Biol       Date:  1998-10       Impact factor: 4.076

7.  A Sulfonylurea Herbicide Resistance Gene from Arabidopsis thaliana as a New Selectable Marker for Production of Fertile Transgenic Rice Plants.

Authors:  Z Li; A Hayashimoto; N Murai
Journal:  Plant Physiol       Date:  1992-10       Impact factor: 8.340

8.  Integration of an insertion-type transferred DNA vector from Agrobacterium tumefaciens into the Saccharomyces cerevisiae genome by gap repair.

Authors:  E Risseeuw; M E Franke-van Dijk; P J Hooykaas
Journal:  Mol Cell Biol       Date:  1996-10       Impact factor: 4.272

9.  The Bean Seed Storage Protein [beta]-Phaseolin Is Synthesized, Processed, and Accumulated in the Vacuolar Type-II Protein Bodies of Transgenic Rice Endosperm.

Authors:  Z. Zheng; K. Sumi; K. Tanaka; N. Murai
Journal:  Plant Physiol       Date:  1995-11       Impact factor: 8.340

10.  Production of two highly active bacterial phytases with broad pH optima in germinated transgenic rice seeds.

Authors:  Chwan-Yang Hong; Kuo-Joan Cheng; Tung-Hai Tseng; Chang-Sheng Wang; Li-Fei Liu; Su-May Yu
Journal:  Transgenic Res       Date:  2004-02       Impact factor: 2.788
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