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Literature DB >> 16668300

Western blot analysis of cereal grain prolamins using an antibody to carboxyl-linked indoleacetic Acid.

Abstract

A monoclonal antibody raised against carboxyl-linked IAA was used in Western blot analysis of storage proteins from kernels of Avena sativa, Pennisetum americanum, Sorghum bicolor, and Zea mays. IAA or an IAA-like molecule is associated with the ethanolsoluble protein fraction of the seed. Western blotting of commercial zein, the major storage protein of maize, along with physicochemical evidence reported by Leverone et al. ([1991] Plant Physiol, 96: 1070-1075) indicated that IAA is linked with this prolamin. Results suggest that an IAA-prolamin association may be widespread throughout the Poaceae.

Entities: Chemical Species

Year: 1991 PMID： 16668300 PMCID： PMC1080895 DOI： 10.1104/pp.96.4.1076

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

11 in total

1. The Release of Auxin from Isolated Leaf Proteins of Spinach by Enzymes.

Authors: S G Wildman; S A Gordon
Journal: Proc Natl Acad Sci U S A Date: 1942-06 Impact factor: 11.205

2. The opaque-2 mutation of maize differentially reduces zein gene transcription.

Authors: R Kodrzycki; R S Boston; B A Larkins
Journal: Plant Cell Date: 1989-01 Impact factor: 11.277

3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

4. Preparation of monoclonal antibodies: strategies and procedures.

Authors: G Galfrè; C Milstein
Journal: Methods Enzymol Date: 1981 Impact factor: 1.600

5. Evidence of zein-bound indoleacetic Acid using gas chromatography-selected ion monitoring-mass spectrometry analysis and immunogold labeling.

Authors: L A Leverone; W Kossenjans; K Jayasimihulu; J L Caruso
Journal: Plant Physiol Date: 1991-08 Impact factor: 8.340

6. Indole-3-acetic Acid (IAA) and IAA Conjugates Applied to Bean Stem Sections: IAA Content and the Growth Response.

Authors: K Bialek; W J Meudt; J D Cohen
Journal: Plant Physiol Date: 1983-09 Impact factor: 8.340

7. Isolation and Partial Characterization of the Major Amide-Linked Conjugate of Indole-3-Acetic Acid from Phaseolus vulgaris L.

Authors: K Bialek; J D Cohen
Journal: Plant Physiol Date: 1986-01 Impact factor: 8.340

2. Evidence of zein-bound indoleacetic Acid using gas chromatography-selected ion monitoring-mass spectrometry analysis and immunogold labeling.

Authors: L A Leverone; W Kossenjans; K Jayasimihulu; J L Caruso
Journal: Plant Physiol Date: 1991-08 Impact factor: 8.340

2 in total

Western blot analysis of cereal grain prolamins using an antibody to carboxyl-linked indoleacetic Acid.

1. The Release of Auxin from Isolated Leaf Proteins of Spinach by Enzymes.

2. The opaque-2 mutation of maize differentially reduces zein gene transcription.

3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

4. Preparation of monoclonal antibodies: strategies and procedures.

5. Evidence of zein-bound indoleacetic Acid using gas chromatography-selected ion monitoring-mass spectrometry analysis and immunogold labeling.

6. Indole-3-acetic Acid (IAA) and IAA Conjugates Applied to Bean Stem Sections: IAA Content and the Growth Response.

7. Isolation and Partial Characterization of the Major Amide-Linked Conjugate of Indole-3-Acetic Acid from Phaseolus vulgaris L.

8. Esters of indole-3-acetic Acid from Avena seeds.

9. Genetic regulation of storaage protein content in maize endosperm.

10. Zein synthesis in maize endosperm by polyribosomes attached to protein bodies.

1. Indole acetic acid distribution coincides with vascular differentiation pattern during Arabidopsis leaf ontogeny.

2. Evidence of zein-bound indoleacetic Acid using gas chromatography-selected ion monitoring-mass spectrometry analysis and immunogold labeling.