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Literature DB >> 16664615

Isolation and Partial Characterization of the Major Amide-Linked Conjugate of Indole-3-Acetic Acid from Phaseolus vulgaris L.

Abstract

A major indole-3-acetic acid conjugate from Phaseolus vulgaris seed has been isolated and partially characterized. It is a 3 kilodalton peptide with apparently 2 indole-3-acetyl moieties in amide linkage per peptide. The indole-3-acetic acid component was identified by gas chromatography-mass spectrometry and the peptide characterized by polyacrylamide gel electrophoresis, by amino acid analysis using dabsyl derivatives and by its Fourier transform-infrared spectrum. This is the first higher molecular weight amide-linked indole-3-acetic acid conjugate to be characterized from higher plants.

Entities: Chemical Species

Year: 1986 PMID： 16664615 PMCID： PMC1075063 DOI： 10.1104/pp.80.1.99

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

19 in total

1. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors: M M Bradford
Journal: Anal Biochem Date: 1976-05-07 Impact factor: 3.365

2. Effect of Endosperm Removal on 7 Normal NaOH-Labile Indole-3-acetic Acid Conjugates in Shoots and Roots of Zea mays Seedlings.

Authors: Y S Momonoki; R S Bandurski
Journal: Plant Physiol Date: 1984-05 Impact factor: 8.340

3. Chromophoric labeling of amino acids with 4-dimethylaminoazobenzene-4'-sulfonyl chloride.

Authors: J K Lin; J Y Chang
Journal: Anal Chem Date: 1975-08 Impact factor: 6.986

4. A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250.

Authors: J J Sedmak; S E Grossberg
Journal: Anal Biochem Date: 1977-05-01 Impact factor: 3.365

5. Photo-regulation of the ratio of ester to free indole-3-acetic acid.

Authors: R S Bandurski; A Schulze; J D Cohen
Journal: Biochem Biophys Res Commun Date: 1977-12-21 Impact factor: 3.575

6. Indole-3-acetic Acid (IAA) and IAA Conjugates Applied to Bean Stem Sections: IAA Content and the Growth Response.

Authors: K Bialek; W J Meudt; J D Cohen
Journal: Plant Physiol Date: 1983-09 Impact factor: 8.340

7. Purification and Partial Characterization of a Glucan Containing Indole-3-acetic Acid.

Authors: Z Piskornik; R S Bandurski
Journal: Plant Physiol Date: 1972-07 Impact factor: 8.340

8. Bound indoleacetic Acid in Avena coleoptiles.

Authors: A Winter; K V Thimann
Journal: Plant Physiol Date: 1966-02 Impact factor: 8.340

9. Bound auxin formation in growing stems.

Authors: P J Davies
Journal: Plant Physiol Date: 1976-02 Impact factor: 8.340

10. The van urk-Salkowski reagent--a sensitive and specific chromogenic reagent for silica gel thin-layer chromatographic detection and identification of indole derivatives.

Authors: A Ehmann
Journal: J Chromatogr Date: 1977-02-11

29 in total

8. Amide-Linked Indoleacetic Acid Conjugates May Control Levels of Indoleacetic Acid in Germinating Seedlings of Phaseolus vulgaris.

Authors: K Bialek; J D Cohen
Journal: Plant Physiol Date: 1992-12 Impact factor: 8.340

9. Metabolism of Auxin in Tomato Fruit Tissue: Formation of High Molecular Weight Conjugates of Oxindole-3-Acetic Acid via the Oxidation of Indole-3-Acetylaspartic Acid.

Authors: J Riov; F Bangerth
Journal: Plant Physiol Date: 1992-11 Impact factor: 8.340

Review 10. Auxin biosynthesis and storage forms.

Authors: David A Korasick; Tara A Enders; Lucia C Strader
Journal: J Exp Bot Date: 2013-04-11 Impact factor: 6.992

Isolation and Partial Characterization of the Major Amide-Linked Conjugate of Indole-3-Acetic Acid from Phaseolus vulgaris L.

1. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

2. Effect of Endosperm Removal on 7 Normal NaOH-Labile Indole-3-acetic Acid Conjugates in Shoots and Roots of Zea mays Seedlings.

3. Chromophoric labeling of amino acids with 4-dimethylaminoazobenzene-4'-sulfonyl chloride.

4. A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250.

5. Photo-regulation of the ratio of ester to free indole-3-acetic acid.

6. Indole-3-acetic Acid (IAA) and IAA Conjugates Applied to Bean Stem Sections: IAA Content and the Growth Response.

7. Purification and Partial Characterization of a Glucan Containing Indole-3-acetic Acid.

8. Bound indoleacetic Acid in Avena coleoptiles.

9. Bound auxin formation in growing stems.

10. The van urk-Salkowski reagent--a sensitive and specific chromogenic reagent for silica gel thin-layer chromatographic detection and identification of indole derivatives.

Review 1. Biosynthesis, conjugation, catabolism and homeostasis of indole-3-acetic acid in Arabidopsis thaliana.

2. Rethinking Auxin Biosynthesis and Metabolism.

Review 3. Biosynthesis, conjugation, catabolism and homeostasis of indole-3-acetic acid in Arabidopsis thaliana.

4. Strawberry fruit protein with a novel indole-acyl modification.

Review 5. Auxin: regulation, action, and interaction.

6. Metabolism of Indole-3-Acetic Acid by Pericarp Discs from Immature and Mature Tomato (Lycopersicon esculentum Mill).

7. Auxin Biosynthesis during Seed Germination in Phaseolus vulgaris.

8. Amide-Linked Indoleacetic Acid Conjugates May Control Levels of Indoleacetic Acid in Germinating Seedlings of Phaseolus vulgaris.

9. Metabolism of Auxin in Tomato Fruit Tissue: Formation of High Molecular Weight Conjugates of Oxindole-3-Acetic Acid via the Oxidation of Indole-3-Acetylaspartic Acid.

Review 10. Auxin biosynthesis and storage forms.