Literature DB >> 16666735

A Simple Purification of Indole-3-Acetic Acid and Abscisic Acid for GC-SIM-MS Analysis by Microfiltration of Aqueous Samples through Nylon.

J R Dunlap1, G Guinn.   

Abstract

A simple procedure was developed for the partial purification of plant tissue samples to be analyzed simultaneously for indole-3-acetic acid (IAA) and abscisic acid (ABA). The procedure relies on removal of contaminants by filtration through nylon and partitioning into dichloromethane. This procedure successfully purified both IAA and ABA from muskmelon, cotton, and broccoli tissue. Twenty individual samples can be purified and methylated in 8 h for analysis of free IAA and ABA with gas chromatography-selected ion monitoring-mass spectrometry. The use of microfiltration of aqueous samples through nylon offers new opportunities for improving the efficiency of existing sample purification procedures.

Entities:  

Year:  1989        PMID： 16666735      PMCID： PMC1061698          DOI： 10.1104/pp.90.1.197

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  6 in total

1.  Comparison of a commercial ELISA assay for indole-3-acetic Acid at several stages of purification and analysis by gas chromatography-selected ion monitoring-mass spectrometry using a c(6)-labeled internal standard.

Authors:  J D Cohen; M G Bausher; K Bialek; J G Buta; G F Gocal; L M Janzen; R P Pharis; A N Reed; J P Slovin
Journal:  Plant Physiol       Date:  1987-08       Impact factor: 8.340

2.  Purification and measurement of abscisic Acid and indoleacetic Acid by high performance liquid chromatography.

Authors:  G Guinn; D L Brummett; R C Beier
Journal:  Plant Physiol       Date:  1986-08       Impact factor: 8.340

3.  C(6)-[benzene ring]-indole-3-acetic Acid: a new internal standard for quantitative mass spectral analysis of indole-3-acetic Acid in plants.

Authors:  J D Cohen; B G Baldi; J P Slovin
Journal:  Plant Physiol       Date:  1986-01       Impact factor: 8.340

4.  A Rapid and Simple Procedure for Purification of Indole-3-Acetic Acid Prior to GC-SIM-MS Analysis.

Authors:  K H Chen; A N Miller; G W Patterson; J D Cohen
Journal:  Plant Physiol       Date:  1988-03       Impact factor: 8.340

5.  ABA Levels and Sensitivity in Developing Wheat Embryos of Sprouting Resistant and Susceptible Cultivars.

Authors:  M Walker-Simmons
Journal:  Plant Physiol       Date:  1987-05       Impact factor: 8.340

6.  Simultaneous quantitation of indole 3-acetic Acid and abscisic Acid in small samples of plant tissue by gas chromatography/mass spectrometry/selected ion monitoring.

Authors:  J H Vine; D Noiton; J A Plummer; C Baleriola-Lucas; M G Mullins
Journal:  Plant Physiol       Date:  1987-10       Impact factor: 8.340
  6 in total
  7 in total

Review 1.  Quo vadis plant hormone analysis?

Authors:  Danuše Tarkowská; Ondřej Novák; Kristýna Floková; Petr Tarkowski; Veronika Turečková; Jiří Grúz; Jakub Rolčík; Miroslav Strnad
Journal:  Planta       Date:  2014-03-28       Impact factor: 4.116

2.  The consequence of peroxidase overexpression in transgenic plants on root growth and development.

Authors:  L M Lagrimini; R J Joly; J R Dunlap; T T Liu
Journal:  Plant Mol Biol       Date:  1997-03       Impact factor: 4.076

3.  Abscisic Acid Alters the Metabolism of Indole-3-Acetic Acid in Senescing Flowers of Cucumis melo L.

Authors:  J R Dunlap; K M Robacker
Journal:  Plant Physiol       Date:  1990-11       Impact factor: 8.340

4.  Influence of water deficits on the abscisic Acid and indole-3-acetic Acid contents of cotton flower buds and flowers.

Authors:  G Guinn; J R Dunlap; D L Brummett
Journal:  Plant Physiol       Date:  1990-07       Impact factor: 8.340

5.  Seasonal changes in needle water content and needle ABA concentration of Japanese red pine, Pinus densiflora, in declining forests on Mt. Gokurakuji, Hiroshima prefecture, Japan.

Authors:  Atsushi Kume; Yuko T Hanba; Kaneyuki Nakane; Naoki Sakurai; Hiroshi Sakugawa
Journal:  J Plant Res       Date:  2006-04-11       Impact factor: 2.629

6.  A Microscale Technique for Gas Chromatography-Mass Spectrometry Measurements of Picogram Amounts of Indole-3-Acetic Acid in Plant Tissues.

Authors:  A. Edlund; S. Eklof; B. Sundberg; T. Moritz; G. Sandberg
Journal:  Plant Physiol       Date:  1995-07       Impact factor: 8.340

7.  Phytoplasma-Responsive microRNAs Modulate Hormonal, Nutritional, and Stress Signalling Pathways in Mexican Lime Trees.

Authors:  Farveh Ehya; Aboozar Monavarfeshani; Ehsan Mohseni Fard; Laleh Karimi Farsad; Mojtaba Khayam Nekouei; Mohsen Mardi; Ghasem Hosseini Salekdeh
Journal:  PLoS One       Date:  2013-06-18       Impact factor: 3.240
  7 in total

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