Literature DB >> 12228526

A Microscale Technique for Gas Chromatography-Mass Spectrometry Measurements of Picogram Amounts of Indole-3-Acetic Acid in Plant Tissues.

A. Edlund1, S. Eklof, B. Sundberg, T. Moritz, G. Sandberg.   

Abstract

A microscale technique has been developed for routine quantifications of picogram amounts of indole-3-acetic acid (IAA) in plant tissues by combined gas chromatography-mass spectrometry. Low- and high-resolution selected-ion-monitoring and selected-reaction-monitoring mass spectrometry techniques were compared for selectivity and precision. The best selectivity was obtained with selected-reaction-monitoring analysis, and 1-mg samples containing 500 fg of IAA could be analyzed accurately with this method. This technique was used to investigate the IAA distribution pattern along the longitudinal axis of tobacco (Nicotiana tabacum [L.]) leaves. In young, developing leaves an increase of endogenous IAA from the leaf tip to the base of the leaf was observed, whereas the level of IAA was uniform along this axis in mature leaves.

Entities:  

Year:  1995        PMID： 12228526      PMCID： PMC157455          DOI： 10.1104/pp.108.3.1043

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  7 in total

1.  Comparison of a commercial ELISA assay for indole-3-acetic Acid at several stages of purification and analysis by gas chromatography-selected ion monitoring-mass spectrometry using a c(6)-labeled internal standard.

Authors:  J D Cohen; M G Bausher; K Bialek; J G Buta; G F Gocal; L M Janzen; R P Pharis; A N Reed; J P Slovin
Journal:  Plant Physiol       Date:  1987-08       Impact factor: 8.340

2.  Selected metastable peak monitoring: a new specific technique in quantitative gas chromatography mass spectrometry.

Authors:  S J Gaskell; D S Millington
Journal:  Biomed Mass Spectrom       Date:  1978-09

3.  A comparison of calculation procedures for isotope dilution determinations using gas chromatography mass spectrometry.

Authors:  B N Colby; M W McCaman
Journal:  Biomed Mass Spectrom       Date:  1979-06

4.  C(6)-[benzene ring]-indole-3-acetic Acid: a new internal standard for quantitative mass spectral analysis of indole-3-acetic Acid in plants.

Authors:  J D Cohen; B G Baldi; J P Slovin
Journal:  Plant Physiol       Date:  1986-01       Impact factor: 8.340

5.  A Simple Purification of Indole-3-Acetic Acid and Abscisic Acid for GC-SIM-MS Analysis by Microfiltration of Aqueous Samples through Nylon.

Authors:  J R Dunlap; G Guinn
Journal:  Plant Physiol       Date:  1989-05       Impact factor: 8.340

6.  Simultaneous quantitation of indole 3-acetic Acid and abscisic Acid in small samples of plant tissue by gas chromatography/mass spectrometry/selected ion monitoring.

Authors:  J H Vine; D Noiton; J A Plummer; C Baleriola-Lucas; M G Mullins
Journal:  Plant Physiol       Date:  1987-10       Impact factor: 8.340

7.  Dual metastable peak monitoring: application to the analysis of oestradiol-17 beta as the bis (tert-butyldimethylsilyl) ether.

Authors:  G C Thorne; S J Gaskell
Journal:  Biomed Mass Spectrom       Date:  1985-01
  7 in total
  73 in total

1.  Control of axillary bud initiation and shoot architecture in Arabidopsis through the SUPERSHOOT gene.

Authors:  T Tantikanjana; J W Yong; D S Letham; M Griffith; M Hussain; K Ljung; G Sandberg; V Sundaresan
Journal:  Genes Dev       Date:  2001-06-15       Impact factor: 11.361

2.  FLOOZY of petunia is a flavin mono-oxygenase-like protein required for the specification of leaf and flower architecture.

Authors:  Rafael Tobeña-Santamaria; Mattijs Bliek; Karin Ljung; Göran Sandberg; Joseph N M Mol; Erik Souer; Ronald Koes
Journal:  Genes Dev       Date:  2002-03-15       Impact factor: 11.361

3.  Auxin as a positional signal in pattern formation in plants.

Authors:  C Uggla; T Moritz; G Sandberg; B Sundberg
Journal:  Proc Natl Acad Sci U S A       Date:  1996-08-20       Impact factor: 11.205

Review 4.  Biosynthesis, conjugation, catabolism and homeostasis of indole-3-acetic acid in Arabidopsis thaliana.

Authors:  Karin Ljung; Anna K Hull; Mariusz Kowalczyk; Alan Marchant; John Celenza; Jerry D Cohen; Göran Sandberg
Journal:  Plant Mol Biol       Date:  2002 Jun-Jul       Impact factor: 4.076

5.  Auxin transport in maize roots in response to localized nitrate supply.

Authors:  Jinxin Liu; Xia An; Lei Cheng; Fanjun Chen; Juan Bao; Lixing Yuan; Fusuo Zhang; Guohua Mi
Journal:  Ann Bot       Date:  2010-10-07       Impact factor: 4.357

Review 6.  Biosynthesis, conjugation, catabolism and homeostasis of indole-3-acetic acid in Arabidopsis thaliana.

Authors:  Karin Ljun; Anna K Hul; Mariusz Kowalczyk; Alan Marchant; John Celenza; Jerry D Cohen; Göran Sandberg
Journal:  Plant Mol Biol       Date:  2002-09       Impact factor: 4.076

7.  Auxin regulation of cytokinin biosynthesis in Arabidopsis thaliana: a factor of potential importance for auxin-cytokinin-regulated development.

Authors:  Anders Nordström; Petr Tarkowski; Danuse Tarkowska; Rikke Norbaek; Crister Astot; Karel Dolezal; Göran Sandberg
Journal:  Proc Natl Acad Sci U S A       Date:  2004-05-14       Impact factor: 11.205

8.  Auxin is required for leaf vein pattern in Arabidopsis.

Authors:  L E Sieburth
Journal:  Plant Physiol       Date:  1999-12       Impact factor: 8.340

9.  Localization of the auxin permease AUX1 suggests two functionally distinct hormone transport pathways operate in the Arabidopsis root apex.

Authors:  R Swarup; J Friml; A Marchant; K Ljung; G Sandberg; K Palme; M Bennett
Journal:  Genes Dev       Date:  2001-10-15       Impact factor: 11.361

10.  Altered life cycle in Arabidopsis plants expressing PsUGT1, a UDP-glucuronosyltransferase-encoding gene from pea.

Authors:  Ho-Hyung Woo; Kym F Faull; Ann M Hirsch; Martha C Hawes
Journal:  Plant Physiol       Date:  2003-08-21       Impact factor: 8.340
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