Studies on the mode of action of acifluorfen-methyl in nonchlorophyllous soybean cells : accumulation of tetrapyrroles.

Phytotoxic effects of the herbicide acifluorfen-methyl on nonchlorophyllous soybean cells were estimated by (86)Rb leakage. An action spectrum study showed maximum injury at 350 to 450 nanometers, with lesser activity between 450 and 700 nanometers. Cells treated in the dark with acifluorfen-methyl accumulated fluorescent pigments with the spectral characteristics of protoporphyrin IX. The action spectrum of acifluorfen-methyl matched the absorption spectrum of this tetrapyrrole, and the extent of cellular damage in the light was related to the degree of fluorescent pigment accumulation. We propose that the phytotoxicity of diphenyl ether herbicides could be explained by their ability to cause abnormal accumulations of tetrapyrroles, which in turn induce lethal photooxidative reactions.