Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Methylation Pattern of Radish (Raphanus sativus) Nuclear Ribosomal RNA Genes.

Literature DB >> 16663896

Methylation Pattern of Radish (Raphanus sativus) Nuclear Ribosomal RNA Genes.

Abstract

The methylation pattern of radish Raphanus sativus nuclear rDNA has been investigated using the Hpa II, Msp I, and Hha I restriction enzymes. The presence of numerous target sites for these enzymes has been shown using cloned rDNA fragments. A large fraction of the numerous rDNA units are heavily methylated, being completely resistant to Hpa II and Hpa I. However, specific sites are constantly available in another fraction of the units and are therefore unmethylated. The use of different probes allowed us to demonstrate that hypomethylated sites are present in different regions. Major hypomethylated Hha I sites have been mapped in the 5' portion of 25S rRNA coding sequence. Among the hypomethylated fraction, different methylation patterns coexist. It has been possible to demonstrate that methylation patterns are specific for particular units. The Hha I pattern of rDNA in tissues of different developmental stages was analyzed. Evidence for possible tissue specific differences in the methylation pattern is reported.

Entities: Species

Year: 1984 PMID： 16663896 PMCID： PMC1064345 DOI： 10.1104/pp.76.3.627

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

24 in total

10. Identification of a short rDNA spacer sequence highly specific of a tomato line containing Tm-1 gene introgressed from Lycopersicon hirsutum.

Authors: H Levesque; F Vedel; C Mathieu; A G de Courcel
Journal: Theor Appl Genet Date: 1990-11 Impact factor: 5.699

Methylation Pattern of Radish (Raphanus sativus) Nuclear Ribosomal RNA Genes.

1. Heterogeneity of pumpkin ribosomal DNA.

2. The sequence GGCmCGG is resistant to MspI cleavage.

3. The effect of site specific methylation on restriction endonuclease cleavage (update).

4. Transcription and methylation of flax rDNA.

5. Sequence arrangement of a highly methylated satellite DNA of a plant, Scilla: A tandemly repeated inverted repeat.

6. DNAase I sensitivity and methylation of active versus inactive rRNA genes in xenopus species hybrids.

7. Organisation of the 5S RNA genes in flax.

8. Effect of CpG methylation on Msp I.

9. Loss of rDNA methylation accompanies the onset of ribosomal gene activity in early development of X. laevis.

10. Two adjacent genomic zein sequences: structure, organization and tissue-specific restriction pattern.

1. The structure of the large spacer region of the rDNA in Vicia faba and Pisum sativum.

2. Methylated and undermethylated rDNA repeats are interspersed at random in two higher plant species.

3. Unmethylated regions in the intergenic spacer of maize and teosinte ribosomal RNA genes.

4. Ribosomal DNA methylation in a flax genotroph and a crown gall tumour.

5. An under-methylated region in the spacer of ribosomal RNA genes of Lilium henryi.

6. Structural analysis of two length variants of the rDNA intergenic spacer from Eruca sativa.

7. Intergeneric hybridization between Diplotaxis siettiana and crop brassicas for the production of alloplasmic lines.

8. Pattern and degree of methylation in ribosomal RNA genes of Cucurbita pepo L.

9. Predominance and tissue specificity of adenine methylation in rice.

10. Identification of a short rDNA spacer sequence highly specific of a tomato line containing Tm-1 gene introgressed from Lycopersicon hirsutum.