Incorporation of [C]Glucosamine and [C]Mannose into Glycolipids and Glycoproteins in Cotyledons of Pisum sativum L.

Developing pea cotyledons incorporate radioactivity in vivo from [(14)C]glucosamine and [(14)C]mannose into glycolipids and glycoproteins. Several different lipid components are labeled including neutral, ionicnonacidic, and acidic lipids. The acidic lipids labeled in vivo appear similar to the polyisoprenoid lipid intermediates formed in vitro in pea cotyledons. Radioactivity from [(14)C]glucosamine and [(14)C]mannose is also incorporated into glycopeptides. Considerable redistribution of [(14)C]mannose into other glycosyl components found in endogenous glycoproteins is observed. An N-acetylglucosamine to asparagine glycopeptide linkage has been isolated from [(14)C]glucosamine-labeled glycoproteins.