Literature DB >> 16661291

Partial purification and characterization of the mRNA for alpha-amylase from barley aleurone layers.

T J Mozer1.   

Abstract

The poly(A)-containing mRNA from barley aleurone layers pretreated with gibberellic acid has been purified by phenol-chloroform extraction and repeated oligo[d(pT)]-cellulose chromatography. This RNA has been translated in both the wheat germ and reticulocyte lysate in vitro translation systems with greater than 50% of the synthesized protein being alpha-amylase. The mRNA for alpha-amylase has been further purified by dimethylsulfoxide-formamide-sucrose density gradient centrifugation and by gel electrophoresis. By these methods, its molecular weight has been determined to be 580,000.

Entities:  

Year:  1980        PMID： 16661291      PMCID： PMC440433          DOI： 10.1104/pp.65.5.834

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  13 in total

1.  Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.

Authors:  J M Bailey; N Davidson
Journal:  Anal Biochem       Date:  1976-01       Impact factor: 3.365

2.  In vitro and in vivo translation of the ribonucleic acids of a cowpea strain of tobacco mosaic virus.

Authors:  G Bruening; R N Beachy; R Scalla; M Zaitlin
Journal:  Virology       Date:  1976-06       Impact factor: 3.616

3.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors:  W M Bonner; R A Laskey
Journal:  Eur J Biochem       Date:  1974-07-01

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  An efficient mRNA-dependent translation system from reticulocyte lysates.

Authors:  H R Pelham; R J Jackson
Journal:  Eur J Biochem       Date:  1976-08-01

6.  Gibberellic Acid-enhanced synthesis and release of alpha-amylase and ribonuclease by isolated barley and aleurone layers.

Authors:  M J Chrispeels; J E Varner
Journal:  Plant Physiol       Date:  1967-03       Impact factor: 8.340

7.  Interactions between Gibberellic Acid, Ethylene, and Abscisic Acid in Control of Amylase Synthesis in Barley Aleurone Layers.

Authors:  J V Jacobsen
Journal:  Plant Physiol       Date:  1973-01       Impact factor: 8.340

8.  Gibberellic acid causes increased synthesis of RNA which contains poly(A) in barley aleurone tissue.

Authors:  J V Jacobsen; J A Zwar
Journal:  Proc Natl Acad Sci U S A       Date:  1974-08       Impact factor: 11.205

9.  Hormonal control of messenger ribonucleic acid metabolism in barley aleurone layers.

Authors:  D T Ho; J E Varner
Journal:  Proc Natl Acad Sci U S A       Date:  1974-12       Impact factor: 11.205

10.  Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.

Authors:  G Blobel; B Dobberstein
Journal:  J Cell Biol       Date:  1975-12       Impact factor: 10.539
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  24 in total

1.  Alterations in gene expression in sorghum induced by the host-specific toxin from Periconia circinata.

Authors:  T J Wolpert; L D Dunkle
Journal:  Proc Natl Acad Sci U S A       Date:  1983-11       Impact factor: 11.205

2.  In vivo synthesis and processing of cereal lectins.

Authors:  H M Stinissen; W J Peumans; A R Carlier
Journal:  Plant Mol Biol       Date:  1982-12       Impact factor: 4.076

3.  Structural analysis of a maize gene coding for glutathione-S-transferase involved in herbicide detoxification.

Authors:  D M Shah; C M Hironaka; R C Wiegand; E I Harding; G G Krivi; D C Tiemeier
Journal:  Plant Mol Biol       Date:  1986-07       Impact factor: 4.076

4.  Messenger RNA encoding a glutathione-S-transferase responsible for herbicide tolerance in maize is induced in response to safener treatment.

Authors:  R C Wiegand; D M Shah; T J Mozer; E I Harding; J Diaz-Collier; C Saunders; E G Jaworski; D C Tiemeier
Journal:  Plant Mol Biol       Date:  1986-07       Impact factor: 4.076

5.  Isolation and characterisation of cDNA clones for tomato polygalacturonase and other ripening-related proteins.

Authors:  A Slater; M J Maunders; K Edwards; W Schuch; D Grierson
Journal:  Plant Mol Biol       Date:  1985-05       Impact factor: 4.076

6.  Changes in cotyledon mRNA during floral induction of Pharbitis nil CV. Violet.

Authors:  M Lay-Yee; R M Sachs; M S Reid
Journal:  Planta       Date:  1987-05       Impact factor: 4.116

7.  Mapping of beta-tubulin genomic sequences in hexaploid oat (Arena sativa L.).

Authors:  N Mendu; H Rines; C D Silflow
Journal:  Theor Appl Genet       Date:  1993-03       Impact factor: 5.699

8.  Maize glutamine synthetase cDNAs: isolation by direct genetic selection in Escherichia coli.

Authors:  D P Snustad; J P Hunsperger; B M Chereskin; J Messing
Journal:  Genetics       Date:  1988-12       Impact factor: 4.562

9.  The beta-tubulin gene family in Zea mays: two differentially expressed beta-tubulin genes.

Authors:  P J Hussey; N Haas; J Hunsperger; J Larkin; D P Snustad; C D Silflow
Journal:  Plant Mol Biol       Date:  1990-12       Impact factor: 4.076

10.  Cloning and expression of a cDNA encoding a maize glutathione-S-transferase in E. coli.

Authors:  R E Moore; M S Davies; K M O'Connell; E I Harding; R C Wiegand; D C Tiemeier
Journal:  Nucleic Acids Res       Date:  1986-09-25       Impact factor: 16.971
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